慢性乙型肝炎与HLA-DRB1等位基因的相关性研究

目的　探讨慢性乙型肝炎与HLA -DRB1等位基因多态性的相关性 ,寻找慢性乙型肝炎的易感基因或抗性基因 ,为临床诊断和预后评估提供线索。方法　采用聚合酶链反应 -序列特异性引物 ( polymerasechainreaction -sequencespecificprimers ,PCR -SSP)技术 ,检测 5 1例慢性乙型肝炎患者和 5 1例健康对照组的HLA -DRB1等位基因多态性。结果　本地区一般健康人群中以HLA -DRB1 15等位基因最常见 ,频率为 16.67%。慢性乙型肝炎组的HLA -DRB1 0 3等位基因频率为 12 .75 % ,显著高于健康对照人群 3 .92 % ( χ2 =5 .2 0 ,P <0 .0 5 ) ,相对危险度为 3 .5 8。其他等位基因频率在两组间的差异无显著性。结论　HLA -DRB1 0 3等位基因与慢性乙型肝炎的易感性呈相关性 ,可能为本地区慢性乙型肝炎的易感基因。

Study on the relationship between chronic hepatitis B and HLA-DRB1 allele

Objective To explore the association between chronic hepatitis B and the polymorphism of HLA-DRB1 allele.Method The technique of polymerase chain reaction-sequence specific primers (PCR-SSP) was selected to detect the HLA-DRB1 allele in 51 patients with chronic hepatitis B and 51 normal control subjects.Results HLA-DRB1 *15 was the frequenest genotype in the healthy residents of Hefei with the frequency of 16.67%.The frequency of HLA-DRB1 *03 allele was 12.75%, which was significantly higher than the frequency (3.92%) in the normal control subjects (χ 2=5.20,P<0.05),the relative risk (RR) was 3.58.The other genotype frequencies of HLA-DRB1 had no significant difference between two groups.Conclusion HLA-DRB1 *03 allele is closely associated with the susceptibility to chronic hepatitis B,and may be the susceptible gene.