HPV E6／E7 mRNA 检测在葫芦岛地区宫颈癌筛查中的应用

目的：探讨人乳头瘤病毒（HPV）E6/E7 mRNA 检测在宫颈癌筛查中的应用。方法选取于该院妇科治疗的160例患者的超薄液基细胞学检查（thin prep cytology test，TCT）标本，根据患者的检查结果将其分为4个组：正常组（32例）、宫颈上皮内瘤变（CIN）Ⅰ组（44例）、CINⅡ组（67例）、CINⅢ组（17例）。除了 CIN 分级外，又根据不同的病理学类型对患者进行分组。在不同的组别中，比较 HPV E6/E7 mRNA 检测和 HPV DNA 检测阳性率的差异；并比较不同病理学分组间 HPV E6/E7 mRNA 和HPV DNA 水平的差异。结果在不同 CIN 分级的患者及对照组人群中，HPV E6/E7 mRNA 和 HPV-DNA 的阳性率比较，差异均有统计学意义（P ＜0．05）。对不同病理学分组间 HPV E6/E7 mRNA 和 HPV DNA 水平进行比较，差异有统计学意义（P ＜0．05）。结论在宫颈癌的筛查中 HPV E6/E7 mRNA 检测可以作为筛选手段之一，该项检测和细胞学检测联合运用有助于诊断准确性的提高。

HPV E6/E7 mRNA detection applied in the cervical cancer screening in Huludao region

Objective To investigate the human papillomavirus （HPV）E6/E7 mRNA detection used in cervical cancer screen-ing.Methods In the study,160 patients treated in gynaecology department of the hospital were enrolled,whose samples were col-lected for ultra-thin liquid-based cytology（thin prep cytology test,TCT）.According to the TCT results,the patients were divided into 4 groups,including normal group（n=32）,CINⅠ group（n=44）,CINⅡ group（n=67）,CINⅢ group（n=17）.In addition to the CIN classification,according to the type of pathology the patients′groups were also devided.In different groups,the differences be-tween HPV E6/E7 mRNA and HPV DNA positive rate were compared respectively.The differences between HPV E6/E7 mRNA and HPV DNA levels among different pathology groups were also compared.Results In groups of different CIN grades and control group,the positive rate of HPV E6/E7 mRNA and HPV DNA test showed statistically diffrences （P 〈0.05 ）.Compared among different pathologic groups HPV E6/E7 mRNA and HPV DNA levels were statistically significant （P 〈0.05）.Conclusion In cer-vical cancer screening,HPV E6/E7 mRNA screening can be used as a screening method and its combined use with cytological meth-od would be helpful to improve the diagnostic accuracy.