时间分辨免疫荧光法检测乙型肝炎病毒血清标志物的研究

目的：探讨时间分辨免疫荧光法（TRFIA）检测乙型肝炎病毒（HBV）血清标志物的准确性及意义。方法选取2011年1月至2013年1月在该院就诊的乙型肝炎（乙肝）或疑似乙肝患者180例，分别采用TRFIA定量和酶联免疫吸附试验（ELISA）定性两种方法检测患者的临床血清标本的乙型肝炎病毒表面抗体（HBsAb）、乙型肝炎病毒表面抗原（HBsAg）、乙型肝炎病毒e抗原（HBeAg）、乙型肝炎病毒核心抗体（HBcAb）、乙型肝炎病毒e抗体（HBeAb）5个指标，将TRFIA设为治疗组，ELISA设为对照组，通过κ检验对两种方法间的一致程度进行评价。结果采用TRFIA检测HBV血清标志物HBsAg、HBsAb、HBeAg、HBeAb、HBcAb的阳性率分别为47．1％、41．1％、44．4％、58．9％、48．9％，采用ELISA检测的阳性率分别为48．3％、42．2％、42．2％、57．8％、52．2％，两组阳性率差异无统计学意义（P＞0．05）；两种方法检测5个指标的效率均表现高度的一致，其中HBsAb指标表现为极强的一致性，其他指标表现为高度一致。结论在检测HBV血清标志物方面，传统的ELISA可靠性也较高，与TRFIA一致性较高，都具有很高的应用价值。

Comparative study of detection of HBV serum markers by time-resolved fluoroimmunoassay

Objective To investigate the accuracy and significance of the time‐resolved fluoroimmunoassay （TRFIA）in detecting hepatitis B virus serum markers .Methods 180 patients with hepatitis B or suspected hepatitis B in our hospital from January 2011 to January 2013 were selected .The TRFIA and the enzyme linked immunosor‐bent assay（ELISA）were adopted to quantitatively and qualitatively detect 5 serum indexes of hepatitis B surface anti‐body（HBsAb） ,hepatitis B virus surface antigen（HBsAg） ,hepatitis B virus e antigen（HBeAg） ,hepatitis B core anti‐body（HBcAb）and hepatitis B virus e antibody（HBeAb）respectively .TRFIA was taken as the treatment group and ELISA as the control group .The consistency of the two kinds of method was evaluated by the κ‐test .Results The serum positive rates of HBsAg ,HBsAb ,HBeAg ,HBeAb ,HBcAb detected by TRFIA were 47 .1% ,41 .1% ,44 .4% , 58 .9% and 48 .9% respectively ;which detected by ELISA were 48 .3% ,42 .2% ,42 .2% ,57 .8% and 52 .2% respec‐tively ,the differences between the two group had no statistical significance（P＆gt;0 .05）;the efficiency of two methods for detecting these 5 indexes was highly consistent ,in which the HBsAb index showed extremely strong consistent and other indicators showed high consistency .Conclusion In the detection of HBV serum markers ,the traditional ELISA has higher reliability and higher consistency with TRFIA ,The two kinds of method have very high application value .