肝细胞癌患者血清 GP73检测的临床价值

目的：探讨血清 GP73检测在肝细胞癌（HCC）诊断中的临床应用价值。方法应用酶联免疫吸附试验（ELISA）定量测定52例健康者、45例非肝性疾病患者、43例乙型病毒性肝炎患者、20例肝硬化患者和30例 HCC 患者血清标本中 GP73蛋白的表达水平；采用电化学发光法（ECL）检测 HCC 患者血清 AFP 水平，计算 ROC 曲线下面积及 GP73和 AFP 对 HCC 诊断的敏感度和特异性。结果非肝性疾病组（58．57±35．64）ng/mL、乙型肝炎组（70．36±49．88）ng/mL 和肝硬化组（114．47±51．27）ng/mL 以及 HCC 组（251．37±95．6）ng/mL 患者血清中的 GP73水平显著高于健康对照组（34．03±15．20）ng/mL，差异均有统计学意义（P ＜0．05）；HCC 组血清 GP73水平非常显著高于其他各组，差异均有统计学意义（P ＜0．01）；HCC 组血清 GP73的阳性检测率（76．7％）显著高于非 HCC 组（3．12％），差异有统计学意义（χ2＝108，P ＜0．05）；GP73诊断 HCC 敏感度（75．1％）和特异性（94．9％）均高于 AFP 的敏感度（52．3％）和特异性（86．6％）。结论 GP73可能成为 HCC 诊断的一个更好的血清标志物。

Clinical value of serum GP73 detection in patients with hepatocellular carcinoma

Objective To explore the clinical application value of the serum GP73 detection in the diagnosis of hepatocellular car-cinoma（HCC）.Methods The expression level of serum GP73 protein was quantitatively detected by ELISA,including 52 cases of healthy individuals,45 cases of non-hepatic disease,43 cases of viral hepatitis B,20 cases of liver cirrhosis and 30 cases of HCC;The levels of GP73 were compared among the 5 groups.Serum a-fetoprotein（AFP）level in the HCC patients was detected by electro-chemilumnescence（ECL）method.The area of receiver operating curve（ROC）,sensitivity and specificity of GP73 and AFP for diag-nosing HCC were calculated.Results The serum level of GP73 was （58.57±35.64）ng/mL in the non-hepatic disease group, （70.36±49.88）ng/mL in the viral hepatitis B group,（114.47±51.27）ng/mL in the liver cirrhosis group and （251.37±95.6）ng/mL in the HCC group,which in the patients was significantly higher than（34.03±15.20）ng/mL in the healthy control group,the differences had statistical significance（P〈0.05）;The serum GP73 level in the HCC group was significantly higher than that in the other groups with statistical difference（P 〈0.01）;The positive detection rate of serum GP73 in the HCC group was 76.7%,which was significantly higher than 3.12% in the non-HCC group,the difference had statistical significance（χ2 =108,P〈0.05）;The sen-sitivity and specificity of GP73 for diagnosing HCC were 75.1% and 94.9% respectively,which were significantly higher than those of the AFP（52.3% and 86.6%）.Conclusion GP73 could be to become a better serum marker for diagnosing HCC.