C型凝集素受体和Toll样受体在原因不明复发性流产患者蜕膜中的表达

目的:研究原因不明复发性流产(URSA)患者蜕膜中树突细胞(DC)特异性细胞间黏附分子-3结合非整合素因子(DC-SIGN)和甘露糖受体(MR)及Toll样受体2(TLR-2)和TLR-4的表达情况。方法:采集URSA患者及正常妊娠人工流产妇女(对照组)的新鲜蜕膜。采用免疫组化和Western blot法检测蜕膜中DC-SIGN、MR、TLR-2和TLR-4蛋白表达,RT-PCR检测蜕膜中DC-SIGN、MR、TLR-2和TLR-4 mRNA表达;流式细胞检测蜕膜中DC表面表达DC-SIGN、MR、TLR-2和TLR-4的水平。结果:与对照组比较,URSA组蜕膜中及DC表面的TLR-2和TLR-4表达水平明显升高(P0.05,P0.01),DCSIGN表达水平明显降低(P0.01)。两组的MR表达水平比较,差异无统计学意义(P0.05)。结论:相对于正常妊娠妇女,URSA患者蜕膜中及树突细胞表面的C型凝集素受体(CLRs)弱势表达,而TLRs优势表达。CLRs与TLRs之间的免疫平衡失调可能是URSA发病机制中的重要因素之一。

Expression of C-type lectin receptors and Toll-like receptors in decidua of unexplained recurrent spontaneous abortion. Expression of C-type lectin receptors and Toll-like receptors in decidua of unexplained recurrent spontaneous abortion

Objective: To investigate the expression of C-typed lectin receptors( CLRs)of DC-SIGN and MR and Toll-like receptors( TLRs) of TLR-2 and TLR-4 in decidua of unexplained recurrent spontaneous abortion( URSA) patients. Methods: The decidua tissues were obtained from URSA patients( URSA group,n = 20) and normal pregnant women( NP group,n= 20).The expressions of DCSIGN,MR,TLR-2 and TLR-4 protein in decidua were detected by immunohistochemistry and Western blot.The levels of DCSIGN,MR,TLR-2 and TLR-4 mRNA in decidua were detected by RT-PCR.The expressions of DCSIGN,MR,TLR-2 and TLR-4 molecule on DC were detected by flow cytometry.Results: The expressions of TLR-2 and TLR-4 on DC and in decidua in URSA group were higher than that in NP group( P<0.05 and P<0.01 respectively).The expression of DC-SIGN in URSA group was significantly lower than that in NP group( P < 0. 01). There was no significant difference in the expression of MR between two groups( P>0.05).Conclusion: Compared with normal pregnant women,the weak expression of CLRs and the overexpression of TLRs were observed in decidua in URSA patients. The imbalance between CLRs and TLRs may play an important role in the pathogenesis of URSA.