| 2型糖尿病大鼠肝脏肝细胞核因子-4α表达的变化 |
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| 引用本文： | 刘东慧,侯丽娜,乔志燕,陈志宏. 2型糖尿病大鼠肝脏肝细胞核因子-4α表达的变化[J]. 承德医学院学报, 2015, 0(2): 100-102 |
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| 作者姓名： | 刘东慧 侯丽娜 乔志燕 陈志宏 |
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| 作者单位： | 1. 承德医学院人体解剖学教研室,河北承德,067000;2. 承德医学院附属医院 |
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| 基金项目： | 国家自然科学基金项目，河北省自然科学基金项目，河北省高校重点发展学科“人体解剖与组织胚胎学”建设项目资助 |
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| 摘 要： | 目的：：探讨2型糖尿病大鼠肝脏肝细胞核因子-4α(HNF-4α)表达的变化和意义。方法：24只雄性SD大鼠随机分为正常对照组和糖尿病模型组，每组12只大鼠。链脲佐菌素连续腹腔注射法制作2型糖尿病大鼠模型。采用SP免疫组织化学染色、Western Blotting法、RT-PCR法检测大鼠肝脏HNF-4α的表达情况。结果：与正常对照组大鼠比较，糖尿病模型组大鼠肝脏HNF-4α蛋白和mRNA的表达均明显升高(P＜0.01)。结论：肝脏HNF-4α表达的升高可能参与了2型糖尿病时肝脏损伤的发生发展。
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| 关 键 词： | 2型糖尿病 肝脏 肝细胞核因子-4α(HNF-4α) |
CHANGES OF HEPATOCYTE NUCLEAR FACTOR 4 ALPHA EXPRESSION IN LIVER OF TYPE 2 DIABETES MELLITUS RATS |
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| Affiliation: | LIU Dong-hui;HOU Li-na;QIAO Zhi-yan;Department of Human Anatomy, Chengde Medical College; |
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| Abstract: | [ABSTRACT]Objective:To investigate changes and signiifcance of hepatocyte nuclear factor 4 alpha (HNF-4α) in liver of type 2 diabetes mellitus rats model.Methods:24 male SD rats were randomly divided into normal control group and diabetes model group with 12 rats in each group. The type 2diabetes model rats were induced by continuous intraperitoneal injection of streptozotocin. SP immunohistochemical stain, Western Blotting and RT-PCR were used to detect HNF-4αexpression in liver.Results: Compared with rats in normal control group, the HNF-4α protein and mRNA expression in liver of rats in diabetes model group increased obviously (P<0.01).Conclusions: High expression of HNF-4α may participate development of liver injury during diabetes mellitus. |
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| Keywords: | Type 2 diabetes mellitus Liver Hepatocyte nuclear factor 4 alpha (HNF-4α) |
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