高效液相色谱法测定丹参中丹参酮IIA的含量

目的建立高效液相色谱法测定中药丹参不同部位中丹参酮IIA的含量。方法采用反相高效液相色谱法,选择色谱柱HypersilODSC18柱(4.6mm×250mm,5μm);流动相:甲醇∶水=75∶25;流速为1.2mL/min;柱温为30℃;检测波长为268～270nm。结果丹参酮IIA浓度在1.25～21.45μg/mL(r=0.9997)范围内线性关系良好,平均加样回收率为99.83%,RSD=2.04%(n=6)。结论采用反相高效液相色谱法测定丹参中丹参酮IIA的含量,该方法快速、灵敏、准确,可以为丹参的种植、品质鉴定、扩展药用部位以及控制丹参药材的质量提供测定依据。

The Determination of the Content of Tanshinone IIA in Danshen

Objective To establish a high-performance liquid chromatography to determine the content of Salvia tanshinone IIA in different parts.Methods Take the RP-HPLC to select the column Hypersil ODSC18 column(4.6mm × 250mm,5μm).The mobile phase: methanol: water = 75∶25.The flow rate was 1.2mL/min.The column temperature was 30 degrees Celsius.The wavelength of detection was 268nm ~ 270nm.Results The tanshinone IIA concentration in 1.25 ~ 21.45μg/mL(r = 0.9997) had a good linear relationship,the average recovery was 99.83%,RSD = 2.04%(n = 6).Conclusion By reversed-phase high performance liquid chromatography in the content of tanshinone IIA in Danshen,the method is rapid,sensitive,accurate,and can provide measurement basis for the planted Salvia,characterization,and expansion of medicinal parts of the quality control of Danshen.