急性坏死性胰腺炎犬肠道菌群的变化

为探讨急性死性胰腺炎时肠道菌变化的规律，经主胰管注人牛磺胆酸钠和胰蛋白酶复制犬 ＡＮＰ模型，而后对模型犬肠粘膜及盲肠内容物的菌群进行定性定量分析，对血及脏器作细菌培养。

AN EXPERIMENTAL STUDY ON CHANGES OF INTESTINAL BACTERIOLOGY IN ACUTE NECROTIZING PANCREATITIS IN DOGS

To observe the changes of intestinal bacteriology in acute necrotizing pancreatitis (ANP). Dog ANP model was induced by injection of sodium taurocholate with trypin into the pancreatic duct. All dogs were sacrificed on the seventh postoperative day, mucosal and luminal microflora of intestine were analyzed quantitatively. The blood and organs were collected for culture. The results showed that population levels of E.coli in the intestinal mucosa and the content in cecum of the ANP dogs showed much higher level than those of the controls ( P <0.01 or P <0.05), while bifidobacterium and lactobacillus were decreased significantly ( P <0.01), resulting in reversal of bifidobacterium/ E.coli ratio. Blood levels of endotoxin were 1-2 times higher in ANP group as compare with the controls. The positive rate of blood and organs were 100% in ANP dogs. E.coli were the major bacteria cultured. The results indicated that microecological disturbance could take place after the onset of ANP, which may take an important role on pancreatic infection complicating ANP.