Two new colorimetric methods for early detection of vancomycin and oxacillin resistance in Staphylococcus aureus |
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Authors: | Coban Ahmet Y Bozdogan Bülent Cihan Cigdem Cekic Cetinkaya Ebru Bilgin Kemal Darka Ozge Akgunes Alper Durupinar Belma Appelbaum Peter C |
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Institution: | Department of Pathology, Hershey Medical Center, P.O. Box 850, Hershey, PA 17033, USA. |
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Abstract: | We developed two colorimetric methods for the detection of vancomycin- and oxacillin-resistant Staphylococcus aureus in =6 h: (i) a nitrate reductase assay and (ii) a resazurin microplate method. MICs agreed with results obtained by CLSI methods for oxacillin. However, detection of vancomycin resistance required a larger inoculum. These methods may be recommended for the detection of vancomycin- and oxacillin-resistant S. aureus. |
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