HPLC法测定玛木然止泻胶囊中盐酸小檗碱与没食子酸的含量

目的采用高效液相色谱法测定玛木然止泻胶囊中盐酸小檗碱和没食子酸的含量。方法色谱柱为Ecosil C18色谱柱(250mm×4.6mm,5μm)。盐酸小檗碱流动相为乙腈-0.05mol·L~(-1)磷酸二氢钾溶液(30∶70),检测波长为345nm,流速为1.0mL·min~(-1);没食子酸流动相为甲醇-2mL·L~(-1)磷酸溶液梯度洗脱,检测波长为270nm,流速为1.0mL·min~(-1)。结果盐酸小檗碱和没食子酸分别在0.211~2.109(r=0.999 1)及0.207~2.071μg·mL~(-1)(r=0.999 6)范围内线性关系良好;平均加样回收率分别为98.20%(RSD=2.20%)和102.52%(RSD=1.28%)。结论该方法准确、简便、重复性好,可用于玛木然止泻胶囊的质量控制。

Determination of berberine hydrochloride and gallic acid in Mamuran Zhixie Capsules by HPLC

Objective To determine the contents of berberine hydrochloride and gallic acid in Mamuran Zhixie Capsules by HPLC . Methods The chromatographic column was Ecosil C18 (250 mm × 4 .6 mm ,5 μm) .The mobile phase consisted of acetonitrile‐0 .05 mol · L -1 potassium dihydrogen phosphate (30∶70) .The detection wavelength was set at 345 nm and the flow rate was 1 .0 mL · min-1 for berberine hydrochloride .The mobile phase consisted of methanol‐2 mL · L -1 phosphoric acid solution in a gradient elution mode .The detection wavelength was set at 270 nm and the flow rate was 1 .0 mL · min-1 for gallic acid .Results The meth‐od had good linear relationship within the range of 0 .211‐2 .109 μg · mL -1 for berberine hydrochloride (r=0 .999 1) and 0 .207‐2.071 μg · mL -1 for gallic acid (r=0 .999 6) ,respectively .The average recoveries were 98 .20% (RSD=2 .20% ) and 102 .52%(RSD=1.28% ) ,respectively .Conclusion The method is accurate ,simple and repeatable ,which can be used for the quality control of Mamuran Zhixie Capsules .