血小板衍生生长因子C对体外培养的人卵巢透明细胞癌细胞的增殖和趋化作用

 目的 观察血小板衍生生长因子-C(platelet-derived growth factor-C,PDGF-C)对体外培养的人卵巢透明细胞癌(ovarian clear cell carcinoma,OCCC)ES-2细胞的增殖和趋化作用。方法 体外培养ES-2细胞,添加不同浓度的PDGF-C进行干预,应用CCK8试剂盒法评价细胞增殖情况,流式细胞术检测细胞周期,利用Transwell细胞迁移实验、细胞划痕实验测定细胞迁移能力。结果 与未加PDGF-C的对照组相比,PDGF-C能明显刺激ES-2细胞的增殖,在第4天增殖达高峰,PDGF-C促增殖最佳作用浓度是20 μg/L;经不同浓度PDGF-C的刺激,处于细胞周期G1期的ES-2细胞比例较对照组明显降低(P<0.05),处于S期的ES-2细胞比例显著升高(P<0.05),而处于G₂+M期的细胞比例则无明显变化(P＞0.05)。同时,PDGF-C提高了ES-2细胞的迁移能力。结论 PDGF-C可促进体外培养的ES-2细胞的增殖、迁移能力。

Effect of platelet-derived growth factor-C on proliferation and migration of ovarian cancer cells in vitro

Objective To examine the effect of platelet-derived growth factor-C (PDGF-C) on ovarian clear cell carcinoma cells ES-2 in vitro.Methods ES-2 ceils were cultured with the supplement of different concentrations of PDGF-C (0,10,20 and 40 μg/L).CCK8 assay was used to detect the effect of PDGF-C on cell proliferation.Flow cytometry was used to detect cell cycle phases.Transwell assay and cell scratch test were used to detect cell migration ability.Results PDGF-C could not only significantly induce proliferation of ES-2 compared with the blank control group without PDGF-C,but increase the percentage of the cells in S phase.Additionally,it was found the best concentration of PDGF-C to promote the proliferation of ES-2 ceils was 20 pg/L.With the stimulation of different concentrations of PDGF-C,the proportion of ES-2 cells in G1 and S cell cycle was significantly decreased (P ＜ 0.05) and significantly increased (P ＜ 0.05) respectively,while the proportion of cells in G2 + M phase did not change compared with the control group (P ＞ 0.05).At the same time,PDGF-C increased the migration ability of ES-2 cells.Conclusions PDGF-C can promote the proliferation and migration of ES-2 cells in vitro.