嗅鞘细胞移植与α晶体蛋白联合对大鼠视神经损伤后视功能恢复的促进作用

目的 研究嗅鞘细胞(olfactory ensheathing cells,OECs)移植与α晶体蛋白联合对大鼠视神经损伤后视功能恢复的促进作用.方法 玻璃体腔α晶体蛋白注射,联合视神经损伤近端移植OECs,分为4个实验组,包括α晶体蛋白注射组、OECs移植组、α晶体蛋白注射+OECs移植组、PBS对照组.于视神经损伤并移植OECs+玻璃体腔注射药物后即刻、1周、2周、1个月、2个月、3个月,用全自动眼电生理仪进行闪烁视觉诱发电位(flash-elicited visual-evoked potential,FVEP)检测,分析比较各时间点各组P1波的振幅及潜时.结果 OECs移植组及α晶体蛋白注射+OECs移植组FVEP的P1波振幅于视神经损伤后1周[(8.49±1.19) μv,(9.33 ±2.54) μv]、2周[(8.85±1.92) μv,(9.43±2.41)μv]、1个月[(8.46±1.09) μv,(8.72±1.91)μv]、2个月[(8.12±1.41)μv,(8.48±1.67) μv]、3个月[(7.68±2.56)μv,(8.08±1.47)μv],均显著高于对照组(P＜0.01),二者联合组P1波振幅恢复更为明显.3个实验组FVEP的P1波潜时与对照组比较,均无统计学差异.结论 OECs移植与α晶体蛋白均明显促进视神经损伤后FVEP电生理的恢复,其中,二者联合的作用最为明显.

Synergistic effect of olfactory ensheathing cells and alpha-crystallin on visual function recovery of adult rats after optic nerve injury

Objective To investigate the synergistic effect of olfactory ensheating cells (OECs) and α-crystallin on visual function recovery of nerve injury in adult rats.Methods α-crystallin was injected into the vitreous cavity,and OECs were transplanted to the area of optic nerve injury.The function of optic nerves was assessed by flash-elicited visual-evoked potential(FVEP) at 1 and 2 weeks,1 and 3 months,and immediately after OECs transplantation and α-crystallin injection.Results Compared to PBS,OECs,the combination of OECs and α-crystallin promoted the recovery of the amplitude of P1 wave at 1 week [(8.49 ± 1.19) μv,(9.33 ± 2.54) μv],2 weeks [(8.85 ± 1.92) μv,(9.43 ± 2.41) μv],1 month [(8.46 ± 1.09) μv,(8.72 ± 1.91) μv],2 months [(8.12 ± 1.41) μ v,(8.48 ± 1.67)μv] and 3 months [(7.68 ±-2.56) μv,(8.08 ± 1.47)μv] after optic nerve injury (P＜0.01).The recowery of the amplitude of P1 wave was much more significantin the combination of OECs and α-crystallin group than in other groups.In α-crystallin group,the amplitude of P1 wave partly recovered after 1 and 2 weeks,but decreased after 1 month,and was not significantly different from that of the PBS group after 3 months.The latency of P1 wave did not recover in OECs group,α-crystallin group,or in the combination group.Conclusions Compared to OECs and α-crystallin alone,the effect of the combination of OECs and α-crystallin on functional recovery is significant.