血小板膜外周型苯二氮卓受体在初发脑梗死后抑郁中的作用

 目的：观察卒中后抑郁（PSD）患者血小板膜外周型苯二氮卓受体（PBRs）的变化，探讨PBRs在PSD中的作用。方法：卒中后抑郁组为初发脑梗死后PSD患者43例、脑梗死组为初发脑梗死患者59例、对照组为健康献血者46名。采用Hamilton抑郁量表（HAMD）评定患者抑郁程度。提取外周血血小板膜，应用放射性配基［3H］PK11195结合实验测定PBRs特异结合活性。结果：［3H］PK11195结合活性3组之间有显著差异（P<0.01）。与对照组［（298.2±25.1) pmol/（g protein）］比较，脑梗死组［3H］PK11195结合活性［（1 410.8±41.4） pmol/(g protein)］显著升高（P<0.01）。与脑梗死组比较，PSD组［3H］PK11195结合活性［（361.7±30.6） pmol/g protein］显著降低（P<0.01）。PSD组男性、女性患者之间比较，［3H］PK11195结合活性差异不显著。PSD组［3H］PK11195结合活性与患者病程无显著相关性（r=0.27，P＞0.05），与HAMD评分呈显著负相关（r=-0.44，P＜0.01）。结论:PSD患者血小板膜PBRs结合活性下降, PBRs影响抑郁程度。

Effects of peripheral-type benzodiazepine receptors in platelet membrane on patients with depression after first attack of cerebral infarction

AIM：To evaluate the changes of peripheral-type benzodiazepine receptors (PBRs) in platelet membrane in post-stroke depression (PSD) patients and to investigate the effects of PBRs on PSD. METHODS：Forty-three patients with PSD, fifty-nine patients with first-ever cerebral infarction and fifty-six healthy volunteers participated in this study. Platelet membrane in venous blood was prepared. Binding assay of the radioactive PBRs antagonist ［3H］PK11195 to platelet membrane was performed. RESULTS：A significant difference of ［3H］PK11195 binding was found among the 3 groups (P<0.01). Compared with the healthy volunteers ［(298.2±25.1） pmol/(g protein)］, a highly significant increase in ［3H］PK11195 binding was observed in platelet membrane in the patients with first attack of cerebral infarction ［(1 410.8±41.4） pmol/(g protein), P<0.01］. Compared with the patients with first attack of cerebral infarction, a significant reduction in ［3H］PK11195 binding was detected in platelet membrane in the patients with PSD ［(361.7±30.6) pmol/(g protein), P<0.01］. In the patients with PSD, no significant difference of ［3H］PK11195 binding was found between men and women (P>0.05). ［3H］PK11195 binding was related to the score of Hamilton depression rating scale (r=-0.44, P<0.01) but not to the duration of cerebral infarction (r=0.27，P>0.05). CONCLUSION:PBRs binding activity in platelet membrane decreases in the patients with PSD and affects the degree of depression.