| 青岛地区大肠埃希菌临床株中blaCTX-M和PMQR基因的检测 |
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| 引用本文： | 尉鸿飞,牟晓东,朱元祺等.青岛地区大肠埃希菌临床株中blaCTX-M和PMQR基因的检测[J].中国实验诊断学,2014(3):425-428. |
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| 作者姓名： | 尉鸿飞 牟晓东 朱元祺等 |
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| 作者单位： | [1]齐鲁工业大学山东省微生物工程重点实验室,山东济南250353 [2]中国石油大学（华东）化学工程学院生物工程与技术中心,山东青岛266580 [3]青岛大学医学院附属医院检验科,山东青岛266003 |
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| 基金项目： | 国家自然科学基金项目（31100059）,中央高校基本科研业务费专项资金（10CX04021A）,青岛经济技术开发区重点科技发展计划项目（2013-1-82） |
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| 摘 要： | 目的 调查青岛地区大肠埃希菌临床分离株中β-内酰胺酶基因blaCTX-M和质粒介导的喹诺酮耐药（Plasmid-mediated quinolone resistance,PMQR）基因的流行性.方法 采用Vitek-2compact系统进行菌株鉴定和药物敏感性测定.聚合酶链反应（Polymerase chain reaction,PCR）法检测菌株中存在的blaCTX M基因和各种PMQR基因,并对PCR扩增产物进行DNA序列测定,确定基因类型.结果 83％的大肠埃希菌对头孢菌素类和氟喹诺酮类药物耐药.84％的大肠埃希菌带有blaCTXM基因,38％、23％、3％、6％和5％的大肠埃希菌分别带有aac（6’）-Ib-cr、oqxAB、qn rB、qnrS及qepA基因.60株菌同时含有PMQR和blacTXM基因,其中10株菌同时含有两种PMQR基因及blacTx-M基因,3株菌同时含有三种PMQR基因及blacTxM基因.结论 blacTxM基因和PMQR基因在青岛地区大肠埃希菌临床分离株中的流行性较高,且常共存于同一株细菌中,这将促进该地区临床细菌多重耐药性产生,加重细菌性感染治疗的难度.
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| 关 键 词： | 大肠埃希菌 blaCTX-M 质粒介导的喹诺酮耐药性 PCR |
Detection of blaCTX-M gene and PMQR genes in clinical Escherichia coli isolates from Qingdao |
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| Institution: | WEI Hong-fei ,MUXi-ao dong ,ZHU Yuan-qi ,et al. (1. Shandong Provincial Key Laboratory of Microbial Engineering ,QiLu University of Technology, J inan 250353, China ; 2. Centre for Bioengineering and Biotechnology , China University of Petroleum (East China ), Qingdao 266580, China ; 3. The Affiliated Hospital of Medical College Qingdao University, Qingdao 266003, China) |
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| Abstract: | Objective This study aimed to investigate the prevalence of β-1actamase gene blacTx M and plasmid-mediated quinolone resistance （PMQR） genes in clinical Escherichia coli isolates from Qingdao,China.Methods Strain characterization and antibiotic susceptibility analysis were performed by using Vitek-2 compact system.The presence and types of blaCTX-M and PMQR genes in clinical isolates were determined by polymerase chain reaction （PCR） and DNA sequencing.Results 83％ isolates could be co-resistant to cephalosporins and fluoroquinolones.84 ％ isolates contained the blacTX-M gene.Prevalences of the PMQR genes were：aac （6 ＇）-Ib-cr,38 ％ ; oqxAB,23 ％ ; qnrB,3 ％ ; qnrS,6 ％ and qepA,5 ％.60 isolates simultaneously carried both PMQR and blacTx-M genes,10 carried two PMQR genes and blaCTX-M genes,and 3 carried three PMQR genes and blacTx M genes.Conclusion High prevalence of PMQR and blaCTX-M genes were identified in clinical E.coli isolates from Qingdao,which would promote multidrug resistance widespread in clinical settings and aggravate the difficulty of bacterial infection treatment in Qingdao. |
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| Keywords: | Escherichia coli blaCTX-M plasmid-mediated quinolone resistance PCR |
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