药盒法制备99mTc-RBC的人体内稳定性及内照射剂量研究

目的研究用自制RBC药盒制备的^99mTc—RBC人体内稳定性和内照射辐射吸收剂量。方法4名健康志愿者，分别取肘静脉血1ml，用自制的RBC药盒进行放射性锝标记，每人注射481～555MBq，给药后5min，1，2．5，4，8和24h进行全身显像，测定^99Tc-RBC在人体内的生物分布；计算各器官在各时间点的放射性占注入量的百分数（％ID）；两种方法绘制各脏器时间一放射性计数曲线，计算各脏器的滞留时间，用MIRDOSE3．0软件计算出各受照器官的内照射辐射剂量。结果^99Tc—RBC在人体内较稳定，血池及大血管于给药后5min至8h均可清晰显影；人体内照射辐射吸收剂量估算结果与文献值相近，按一次给药555MBq计算，各器官的吸收剂量均低于50mGy。结论用本实验室自制RBC药盒制备的^99Tc—RBC体内稳定性好，内照射辐射吸收剂量符合要求。

Stability and dosimetry of 99mTc-RBC prepared by kit method in healthy volunteers

Objective To study the in vivo stability of and absorbed radiation dose from 99mTc-RBC, prepared using RBC kit made in our laboratory. Methods Four healthy volunteers were studied with 99mTc-RBC prepared by kit method. Whole body images were acquired at 5 min, 1, 2.5, 4, 8 and 24 hours after intravenous injection of 481-555 MBq 99mTc-RBC. Biodistribution analysis was performed in regions of interest (ROI). The absorbed radiation doses were calculated using the MIRDOSE3.0 analysis program and the time-related activity curves for different organs were used for the residence time calculations. Results 99mTc-RBC prepared by kit method was stable in vivo. The quality of blood pool imaging from 5 min to 8 h in healthy volunteers was satisfactory. The absorbed radiation dose was lower than the permissible value. Conclusion 99mTc-RBC prepared by kit method is very suitable for clinical application.