贵州某白酒对大鼠睾丸间质细胞内类固醇激素急性调节蛋白表达及血清睾酮水平的影响

目的探讨摄入贵州某54°白酒后,不同时程及不同剂量对大鼠睾丸间质细胞内类固醇激素急性调节蛋白(St AR)表达及血清睾酮水平的变化,以期为科学饮酒提供基础资料。方法正常成年雄性SD大鼠69只,随机分为正常对照组(n=15)及实验组(n=54)。实验组分为低剂量组0.8ml/(kg·d)、中剂量组1.6ml/(kg·d)及高剂量组2.4ml/(kg·d),实验组大鼠每日灌胃2次。分别于第4周末、第8周末、第12周末采血清,取睾丸组织。采用免疫组织化学SABC法、图像分析、Western blotting检测睾丸组织St AR的表达,采用化学发光法检测大鼠血清睾酮水平。结果各实验组的血清睾酮水平与正常对照组比较未见明显异常,差异无统计学意义(P0.05)。St AR免疫组织化学阳性产物存在于睾丸间质细胞胞质内;与正常对照组比较,4周、8周、12周各剂量组St AR免疫染色及平均吸光度均有所增强(P0.05);Western blotting检测,4周、8周、12周各剂量组St AR蛋白表达水平增强(P0.05)。结论在本实验设定的剂量和时程内用该白酒灌胃后,大鼠睾丸间质细胞内St AR表达水平有所增强,血清睾酮水平并无明显变化。

Effect of Guizhou liquor on rat Leydig cells in the expression of steroidogenic acute regulatory protein and serum testosterone level

Objective To investigate effects of a Guizhou 54 degrees liquor on rat leydig cells in the expression of steroidogenic acute regulatory protein (StAR) and the change of the serum testosterone level at the different time and with different doses. Methods Sixty normal adult male SD rats were randomly divided into a normal control group(n=15) and an experimental group(n=54). Each experimental group was further subdivided into low dose group 0.8 ml/（kg·d）, middle dose group 1.6 ml/（kg·d）, high dose group 2.4 ml/（kg·d）. The rats of the experimental groups were given by gavage twice a day(11∶00 AM an 5∶00 PM)with liquor. At the end of the 4th, 8th and 12th week, the serum was collected and testicular tissue was taken. The expression of StAR was detected by immunohistochemical SABC method, image analysis and Western blotting, and level of the serum testosterone was detected by Chemiluminescence method. Results StAR immunohistochemical positive products existed within the cytoplasm of Leydig cells; Compared with the normal control group, the average absorbance of StAR staining in the experimental groups at the 4th, 8th, and 12th week increased(P<0.05). Western blotting showed that the expression level of StAR protein in the experimental groups at the 4th, 8th and 12th week increased(P<0.05). Compared with normal control group, serum testosterone level of each experimental group did not see apparently unusual,and there was no statistically significant difference(P>0.05). Conclusion In this experiment a set of doses and schedules after gavage with the liquor enhance the StAR expression level in the rat Leydig cells enhancement.Serum testosterone level has no obvious change.