小鼠脂肪源和骨髓源间充质干细胞表面标记表达的比较

目的探讨小鼠脂肪源间充质干细胞(ADMSCs)和骨髓源间充质干细胞(BMSCs)表面标记表达的差异。方法分别对小鼠脂肪源和骨髓源间充质干细胞进行成骨、成脂、成心肌分化诱导、鉴定;流式细胞术与细胞免疫荧光法检测CD29、CD44、CD45和CD73的表达并进行比较。结果 BMSCs形态均一,呈长梭形;ADMSCs形态多样,呈梭形、星形、多角形等,胞体较大,表面分泌物较多;两者均可诱导分化为成骨细胞、脂肪细胞和心肌样细胞。流式细胞术检测显示,BMSCs表面分子CD29、CD44和CD73表达率分别为(83.43±1.97)%、(90.33±0.81)%、(2.63±0.42)%;ADMSCs表面分子CD29和CD44的表达率分别为(53.1±1.05)%、(34.8±2.1)%,CD73表达不稳定,在1.8%~19.7%之间波动。两种细胞均不表达造血干细胞标记物CD45。免疫荧光显示,CD73分子与部分CD29、CD44阳性细胞共表达。结论小鼠BMSCs和ADMSCs均具有成脂、成骨、成心肌分化能力,但是在形态上和分子表达上均存在差异。BMSCs的CD44和CD29表达高于ADMSCs;而CD73的表达则相反,CD73在两种细胞的表达均较低,在ADMSCs的表达不稳定。

Expression difference of surface markers between adipose derived mesenchymal stem cells and bone marrow mesenchymal stem cells of the mouse

Objective To investigate the expression difference of surface markers of mouse mesenchymal stem cells from adipose tissue and bone marrow. Methods Mouse adipose-derived mesenchymal stem cells（ADMSCs）and bone marrow derived mesenchymal stem cells(BMSCs) were induced to differentiate into osteoblasts, adipocytes and cardiomyocytes and then indentified their differentiation in vitro. The expressions of CD29，CD44，CD45 and CD73 were detected and compared by flow cytometry and immunofluorescence. Results The morphology of BMSCs was uniform，and most of them were spindle. While ADMSCs showed multiplicity，presenting spindle, starlike and polygonal on which were much excretion，and their size was large.Both cells differentiated to osteoclasts, adipocytes and cardiomyocytes-like cells.The result of flow cytometry showed BMSCs were positive for CD29（83.43±1.97）%，CD44（90.33±0.81）%and CD73（2.63±0.42）%；ADMSCs were positive for CD29（53.1±1.05）%，CD44（34.8±2.1）%，while the expression of CD73 was unstable from 1.8% to 19.7%.Both were negative for CD45.The result of Immunofluorescence indicated the co-expression of CD73 and CD29，CD44. Conclusion Both BMSCs and ADMSCs have the potential to differentiate into adipose，osteogenesis，and myocardial muscle.Mouse BMSCs and ADMSCs are different in the cells morphology and the expression of the surface marker；both highly expressed CD29 and CD44，but BMSCs have consistently high expression of CD29 and CD44 compared with ADMSCs.On the contrary，the expression of CD73 is significantly lower on both cells and unstable on ADMSCs.