高强度聚焦超声波对细粒棘球绦虫原头节酶活性的影响

目的探索高强度聚焦超声波(HIFU)对体外分离的细粒棘球绦虫原头节的酶活性的影响作用。方法用不致原头节即刻杀伤的高强度聚焦超声剂量照射原头节,行酶组织化学染色观察酸性磷酸酶(ACP)、葡萄糖6-磷酸酶(G-6-P)和琥珀酸脱氢酶(SDH)的活性,了解高强度聚焦超声对体外培养的原头节3种代谢酶活性的影响作用。结果细粒棘球绦虫原头节具有酸性磷酸酶、葡萄糖6-磷酸酶和琥珀酸脱氢酶活性。不致原头节即刻杀伤的高强度聚焦超声波作用后,辐照组原头节葡萄糖6-磷酸酶和琥珀酸脱氢酶活性较对照组明显减弱,而酸性磷酸酶反应产物与对照组相比无明显变化。阴性对照组无酶反应产物产生。结论高强度聚焦超声波辐照对原头节酸性磷酸酶活性无影响,而对葡萄糖6-磷酸酶和琥珀酸脱氢酶的活性有明显的抑制,这可能是影响原头节的代谢功能,最终抑制其增殖的原因之一。

Effect of high intensity focused ultrasound on the enzymatic activity of Echinococcus granulosus protoscolices

The effect of high intensity focused ultrasound(HIFU)on the enzymatic activity of Echinococcus granulosus protoscolices was investigated,in which the protoscolices of E.granulosus was irradiated with a dosage of HIFU without causing the immediate killing of protoscolices,and the activities of acid phosphayase(ACP),glucose-6-phosphatase(G-6-P)and succinate dehydrogenase(SDH)in E.granulosus protoscolices were determined by histochemical staining methods for enzymes.It was found that after irradiation with a dosage of HiFU without causing the immediate killing of protoscolices,the enzymatic activities of.G-6-P and SDH decreased markedly,but no significant difference in the activity of ACP could be demonstrated between the HIFU-treated and the control group.It is concluded that HIFU shows definite inhibitory effect on the enzymatic activities of G-6-P and SDH,which may be one of the causes resulting in the inhibition of proliferation of E.granulosus protoscolices.