低体温对大鼠血液有形成分的影响

目的探讨低体温对血液有形成分的影响。方法实验动物为健康雄性wistar大鼠，随机分为11个组，Ⅰ组为对照组，Ⅱ～Ⅺ组为实验组。实验组大鼠单笼置寒冷环境模拟实验室中于-30℃环境进行冷暴露，Ⅱ-Ⅳ组为低体温组，分别于-30℃冷暴露30、60、80min；Ⅴ-Ⅺ组为低体温后复温组，于-30℃冷暴露80min后室温自然复温，分别复温4、8、12，16、24、72、120h，采血测定血液有形成分数量。结果-30℃冷暴露30min，血液中WBC、RBC、PLT数量均增多；冷暴露80min3项指标均显著高于对照组（P〈0．05，P〈0．01）；至复温120h，WBC、PLT数量始终较对照组增高，RBC数量呈现波动变化。结论低体温可引起WBC、RBC、PLT3种血液有形成分数量明显变化，为低体温机制的研究和治疗效果的评价提供参考指标。

The Effect of Hypothermia on The Blood Visible Component of Rats

Objective] To explore the effect of hypohermia on the blood visible components of rats. Methods] Ninety healthy male Wistar rats were randomly divided into eleven groups. Group I and group Ⅱ -XI were for control group and experimental group respectively. The rats of experimental groups were exposed to cold condition at the temperature of - 30 ℃, the rats of group Ⅱ -Ⅳ （ hypothermia group ） were exposed over a period of 30 min, 60 min and 80 min respectively. Group V - XI （ rewarming group ） rewarmed in room temperature for 4hours, 8hours, 12hours, 16hours, 24hours, 72hours and 120hours respectively after 80min exposure. The counts of blood visible components collected from the rats were measured. Results] After 30min exposure, the counts of WBC, RBC and PLT increased. After 80rain exposure the counts of WBC, RBC and PLT were significantly higher than those of control groupl P 〈0.05 ,P 〈0.01 ）. After rewarming for 120 hours, the level of WBC, PLT counts were always higher than the control group but RBC count showed fluctuant changes. Conclusion] The significant change of counts of blood visible components such as WBC, RBC, PLT caused by hypothermia provide reference for hypothermia mechanism research and evaluation of treatment effect.