反相高效液相色谱法测定芩蓝滴鼻液中黄芩苷含量

目的建立反相高效液相色谱法分离测定芩蓝滴鼻液中黄芩苷的含量。方法：采用Gemini C18110A（4．60mm×250mm，5μm）色谱柱；流动相为甲醇旬-0．2％磷酸溶液（50：50）；流速为0．8ml／min；检测波长为277nm；进样量10μl。结果黄芩苷浓度在709．4～7094．0μg/ml范围内与峰面积呈良好的线性关系，r=0．9999；平均回收率为99．6％，RSD为1．33％（n=9）。结论本方法简便、准确、专属性强，可用于测定芩蓝滴鼻液中黄芩苷的含量。

Quantitative analysis of baicalin in Qinlan nasal drops by RP-HPLC

Objective To develop RP-HPLC method for the determination of baicalin in Qinlan nasal drops. Methods The chromatographic analysis was performed on Gemini C18 110A（4.60 mm× 250 mm,5 μm）column. The mobile phase was consisted of methanol-0.2% phosphoric acid solution（50 ： 50）. The flow rate was 0.8 ml/min. The detection wavelength was set at 277 nm, with injection volume 10 pJ. Results A good linearity was obtained over the range of 709.4 -7094.0μg/ml （r = 0.999 9）. The average recovery of baicalin was 99.6%, with RSD 1.33 % （n = 9）. Conclusion The method is simple, accurate, specific and will be used for the quality control of Qinlan nasal drops.