肿瘤转移相关基因在卵巢癌和宫颈癌组织中的表达及其临床意义

目的探讨肿瘤转移相关基因编码蛋白在卵巢癌和宫颈癌转移中的表达规律及其临床意义。方法采用流式细胞术对29例卵巢癌和19例宫颈癌患者瘤组织的CD44V5+、CD44V6+、cerbB-2+、nm23+、DCC+和p16+细胞检出率和DNA含量进行了检测和分析。结果卵巢癌和宫颈癌患者瘤组织细胞的CD44V5+、CD44V6+和cerbB-2+细胞表达率均显著高于正常对照组(P<0.01或0.05);nm23+,DCC+和p16+细胞表达率则显著低于正常对照组(P<0.01或0.05)。肿瘤转移者、多器官转移者、DNA异倍体者、SPF增高者、Apo减低者的CD44V5+、CD44V6+、cerbB-2+细胞表达率均显著高于未转移者、单器官转移者、DNA二倍体者、SPF不增高者、APO不减低者(P<0.01或0.05)。而nm23+、DCC+、p16+细胞的表达率与此相反。结论卵巢癌和宫颈癌组织的肿瘤转移相关基因编码蛋白表达水平的检测,对判断肿瘤的恶性度、转移潜能、转移严重程度及患者预后估计有重要价值。

Expression and clinical significance of tumor metastasis related genes in ovarian and cervical cancer tissues

Objective To study the expression regularity and its significance of tumor metastasis related genes in tumor tissues of patients with ovarian or cervical cancer.Methods A flow cytometry was used to detect the expression rates of CD44V5+,CD44V6+,cerbB-2+,nm23+,DCC+,p16+ cells and DNA content in 29 patients with ovarian cancer and 19 patients with cervical cancer,then the data was analyzed.Results The expression rates of CD44V5+,CD44V6+,and cerbB-2+ cells in tumor tissues of patients with ovarian or cervical cancer were higher than those of normal control groups(P<0.01 or 0.05),while the expression rates of nm23+,DCC+,p16+ cells were lower than those of normal control groups(P<0.01 or 0.05).The expression rates of CD44V5+,CD44V6+,cerbB-2+ cells in patients with metastasis,multi-metastasis nodes,DNA heteroploid,SPF elevated,and Apo decreased were higher than those of patients with non-metastasis,only one-metastasis node,DNA diploid,SPF non-elevation and Apo non-decreasing,respectively(P<0.01 or 0.05),while nm23+,DCC23+ and p16+ were contrary.Conclusion Detection of expression level of different tumor metastasis related genes coding protein in ovarian and cervical cancer tissues will be helpful not only to the judgment of the tumor's malignant degree,metastasis potency and metastasis severity,but also to the patients' prognosis estimation.