山东地区新生儿脐血HLA-A、HLA-B等位基因多态性研究

目的研究山东地区新生儿脐血人类白细胞抗原（HLA—A、HLA—B）等位基因的分布特征．探讨国人用山东脐血供者进行造血干细胞移植的可能性。方法应用PCR—SS0方法对山东地区5844例无血缘关系的汉族健康新生儿脐血进行HLA—A、HLA—B等位基因分布调查。结果检出20种HLA—A等位基因，频率较高的为A＊02（0．3041）、A＊11（0．1443）、A＊24（0．1434）、A＊30（0．0975）和A＊33（0．0859），较低的为A＊34（0．0006）、A＊25（0．0005）、A＊66（0．0005）、A＊74（0．0004）和A＊36（0．0001）。检出46种HLA—B等位基因．频率较高的为B＊13（0．1348）、B＊51（0．0713）、B＊62（0．0712）、B＊61（0．0676）和B＊60（0．0642），较低的为B＊77（0．0001）．B＊76（0．0002）．B＊47（0．0003），B＊42（0．0003）和B＊72（0．0004）。结论山东新生儿脐血HLAI类基因具有多态性，能代表山东汉族人群HLA的分布特征，反映北方汉族HLA的分布规律；国人（尤其是北方汉族人）在山东脐血库中最容易找到HLAI类等位基因相合的异基因脐血供者。

Study of HLA-A and HLA-B allele polymorphism in Shandong neonates umbilical cord blood

Objective] To investigate the HLA-A,-B allele polymorphism in Shandong neonates umbilical cord blood and explore the possibility of finding the cord blood donor of HLA-A,B matched to perform the stem cell transplantation for Chinese.Methods] The subjects of the study were drawn from 5844 Shandong unrelated umbilical cord blood samples in Shandong Umbilical Cord Bank and investigated by polymerase chain reaction-sequence specific oligo-nucleotide(PCR-SSO). Results] 20 alleles at HLA-A locus,and 46 alleles at HLA-B locus in Shandong umbilical cord samples were detected.Among 20 HLA-A alleles detected,the most frequent five alleles were A*02(0.3041),A*11(0.1443),A*24(0.1434),A*30(0.0975) and A*33(0.0859).A*34(0.0006),A*25(0.0005,A*66(0.0005),A*74(0.0004) and A*36(0.0001) had lower gene frequency.Among 46 HLA-B alleles detected,the most frequent five alleles were B*13(0.1348),B*51(0.0713),B*62(0.0712),B*61(0.0676)and B*60(0.0642);HLA-B*77(0.0001),B*76(0.0002),B*47(0.0003),B*42(0.0003)and B*72(0.0004) showed lower gene frequency.Conclusion] HLA-A,-B allele in Shandong umbilical cord blood have rich polymorphism,the distributing character of HLA-A,-B allele in Shandong Hans were showed,and the HLA distributing character in Northern Chinese was further reflected.Chinese patients are easier to search HLA-A,-B matched cord blood donors in Shandong Umbilical Cord Blood Bank,especially Northern Han Chinese.