高糖对脑微血管内皮细胞活力和内皮素-1的影响

目的：探讨体外培养条件下高糖状态对大鼠脑皮质微血管内皮细胞（Brain microvascular endothelial cells，BMEC）的影响。方法：建立大鼠脑皮质微血管内皮细胞体外培养模型，用透射电镜观察正常浓度（5．05mmol／L）和高浓度（15mmol／L、30mmol／L）葡萄糖培养基中培养24h后内皮细胞的超微结构变化，用MTT法评价内皮细胞活力，采用放射免疫方法测定内皮素-1（Endothelin-1，ET-1）含量，用原位杂交法测定ET-1mRNA表达。结果：高浓度葡萄糖可使内皮细胞超微结构损伤，细胞活力降低，ET-1分泌显著上调，并呈剂量依赖性损伤细胞，但ET-1mRNA表达无明显变化。结论：高糖对脑皮质微血管内皮细胞有显著的损伤作用，ET-1升高在糖尿病微血管病变早期可能起主要作用。

Effects of high glucose concentration on endothelin-1 and activity of cultured brain microvascular endothelial cells

Objective:To investigate the effects of high glucose on rat brain microvascular endothelial cells(BMEC).Methods: Brain microvascular endothelial cells(BMEC)of Wistar rat cerebral cortex were cultured in 5.05mmol/L glucose,15 and 30mmol/L glucose media respectively for 24h,and then BMEC activity or injury was assayed by observing cell ultramicrostructures morphology and adopting methyl thiazolyl tetrazolium(MTT)assay.The ET-1 concentrations were evaluated by radioimmunoassay.Situ hybridization used to detect the ET-1 mRNA expression.Results:The BMEC activity and viability assessed by MTT were decreased when glucose concentrations rose.The ultrastructure of BMEC was damaged and the content of ET-1 in BMEC media was increased.Furthermore,ET-1 mRNA expression was not induced by high concentration of glucose.Conclusions:High concentration of glucose has a significant effect of BMEC injury.The data suggest that ET-1 may play important role in the early state of diabetic microvascular complications.