米力农对大鼠缺血再灌注损伤中的保护作用

目的探讨米力农对在体肺缺血再灌注（I／R）损伤的保护作用。方法夹闭大鼠左肺门1h，再灌注3h，建立在体缺血再灌注模型。将20只SD大鼠随机分成缺血再灌注组（对照组）；米力农组（实验组）。米力农组缺血前30min给予颈静脉推注米力农1mg／kg。再灌注3h后摘取左肺测超氧化物歧化酶（SOD）活力、丙二醛（MDA）含量及髓过氧化物酶（MPO）活力并行病理学检查。结果再灌注3h后，两组间MPO活力无显著性差异。对照组MDA（nmol／mgprot）含量为0．884±0．06，显著高于实验组0．494±0．11（P〈0．05），而SOD（U／mgprot）活力为16．094±10．73，显著低于实验组35．93±13．50。结论米力农对肺缺血再灌注损伤具有保护作用，可能与其抗氧化作用有关。

Study of protective effect of milrinone on lung injury induced by ischemia/reperfusion

Objective To investigate the protective effect of Milrinone protects against experimental lung ischemia reperfusion injury（ IRI） in a situ hilar clamp model. Methods Left lung of rat was rendered ischemic for 1 hour,and reperfused for up to 3 hours,as the model, twenty SD rats were randomly divided into 2 groups with 10 rats each group：ischemia reperfusion treatment group （control group） , ischemia reperfusion and Milrinone treatment group（experiment group）. Rats of experiment group received Milrinone （ 1 mg/kg） intravenously 30 minutes before ischemia. After 3 hours of reperfusion, the activity of Erythrocuprein （ SOD ） , the content of malonaldehyde（MDA） and the activity of myeloperoxidase （MPO） were determined, and pathematology changes were observed in the left lung tissue. Results After 3 hours of Zperfusion, there were no significant changes of the activity of MPO between two groups. The content of MDA（nmol/mgprot） of control group were 0. 88 ±0. 06, significantly higher than that of the experiment group 0.49±0. 11 （ P 〈 0.05 ）. The activity of SOD （U/mgprot） of control group were 16. 09 ± 10.73 ,significantly lower than that of the experiment group 35.93 ± 13.50 （P 〈 0.05 ）. Conclusion Milrinone protect the lung function against I/R injury, which may be related to resisting oxidation.