Gabl与MMP-9在胆管癌中的表达及意义

目的：探讨胆管癌中Gabl与MMP-9的表达及意义。方法：胆管癌标本70例，胆管癌旁组织30例，正常胆管组织30例，应用免疫组化法检测Gabl和MMP-9蛋白的表达情况。结果：胆管癌组Gabl的平均光密度为0．1689±0．0268，癌旁组为0．1471±0．0185，正常胆管组为0．1023±0．0119，三组结果比较差异有显著性（P〈0．05）；胆管癌组MMP-9的平均光密度为0．1887±0．0289，癌旁组为0．1345±0．0196，正常胆管组为0．0758±0．0104，三组结果比较差异有显著性（P〈0．05）。Gabl和MMP-9在胆管癌中的表达与有无淋巴结转移及肿瘤分期有关（P〈0．05），而与肿瘤大小、分化程度无关（P〉0．05）。Gabl与MMP-9的表达存在正相关（r=0．362，P〈0．05）。结论：Gabl和MMP-9蛋白表达增高与胆管癌的发生、发展及侵袭转移有关；Gabl和MMP-9在胆管癌组织中的表达呈正相关。

Expression and significance of Gab]. and MMP -9 in cholangiocarcinoma

Objective ：To investigate the expression and significance of Gabl and MMP -9 in cholangiocarcinoma. Methods： Total of 70 samples of cholangiocarcinoma, 30 samples of bile duct paracarcinoma tissue, and 30 normal bile duct tissue were chosen. The expression of Gabl and MMP -9 protein was detected by immunohistochemical method. Results： Average optical density of Gabl was 0. 1689 ±0. 0268, 0. 1471±0. 0185, 0. 1023 ±0. 0119 in cholangiocarcinoma group, paracarcinoma group and normal bile duct group （ P 〈 0.05 ）. Average optical density of MMP -9 was 0. 1887 ±0. 0289, 0. 1345 ±0. 0196, 0. 0758 ±0. 0104 in cholangiocarcinoma group, paracarcinoma group and normal bile duct group（P 〈 0.05 ）. The expression of Gabl and MMP- 9 in cholangiocarcinoma was relat- ed with neoplasm staging and lymph node metastasis（P 〈 0.05）, but had no relation with the size and differentiation of tumor（ P 〉 0.05 ）. There was positive correlation between Gabl and MMP - 9 expressions （ r = 0. 362 ,P 〈 0.05 ）. Conclusion： High expression of Gab1 and MMP- 9 has the correlation with occurrence, development, invasion and metastasis of cholangiocarcinoma; There is positive correlation between Gabl and MMP-9 expressions in bile duct carcinoma tissue