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当归多糖及其中性组分对巨噬细胞分泌TNF-α的影响
引用本文:奚瑾磊,彭仁琇,杨哲琼. 当归多糖及其中性组分对巨噬细胞分泌TNF-α的影响[J]. 武汉大学学报(医学版), 2002, 23(1): 21-23
作者姓名:奚瑾磊  彭仁琇  杨哲琼
作者单位:武汉大学医学院药理学教研室,武汉,430071
摘    要:目的 :观察当归多糖 (ASP)及其中性组分 (ASP 1)在体内外对小鼠腹腔巨噬细胞 (MФ)分泌TNF α的影响。方法 :直接制取小鼠腹腔MФ ,与ASP及ASP 1共同培养 16h ;ASP及ASP 112 5、2 5 0mg kg经口灌胃给药 7d ,于第 7d制取MФ ,体外培养 16h ,分别观察ASP及ASP 1在体外均可显著增强MФ对TNF α的分泌。ASP及ASP 1体内给药无直接诱导MФ分泌TNF α的功能。结论 :ASP及ASP 1在体内外均可增强小鼠腹腔MФ分泌TNF α的功能。

关 键 词:当归多糖  巨噬细胞  TNFα
修稿时间:2001-03-25

Effects of Angelica Sinensis Polysaccharides and Its Litmusless Component on TNF-α Produced by Peritoneal Macrophages
Xi Jinlei,Peng Renxiu,Yang zheqiong. Effects of Angelica Sinensis Polysaccharides and Its Litmusless Component on TNF-α Produced by Peritoneal Macrophages[J]. Medical Journal of Wuhan University, 2002, 23(1): 21-23
Authors:Xi Jinlei  Peng Renxiu  Yang zheqiong
Affiliation:Xi Jinlei,Peng Renxiu,Yang zheqiong Department of Pharmacology,School of Medicine,Wuhan University,Wuhan 430071,China
Abstract:Objective:To odserve the effect of Angelica sinensis polysaccharides(ASP) and litmusless component(ASP 1) on TNF α produced by mice peritoneal macrophages. Methods:Mice peritoneal macrophages with no pretreatment were co cultured with ASP or ASP 1 in vitro for 16 hours. After prepared from mice administered with ASP or ASP 1(125?250 mg/kg)for 7 days in vivo, macrophages were cultured for 16 hours. L929 cell line cytotoxicity was used for assay of TNF α secreted by macrophages. Results:The production of TNF α could be significantly promoted by ASP or ASP 1 in vitro.ASP or ASP 1 in vitro.ASP or ASP can not directly induced the macrophages to secret TNF α in vivo,but could enhance hte production of TNF α by macrophages induced by LPS. Conclusions: ASP and ASP 1 can promote macrophages to secret TNF α in vitro and in vivo.
Keywords:Angelica sinensis polysaccharides  macrophages   TNF α 1
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