钩藤碱对缺血-再灌注大鼠脑NOS变化的作用

目的：研究钩藤碱（Ｒｈｙ）对大鼠脑缺血－再灌注皮层和海马一氧化氮合酶（ＮＯＳ）变化的作用。方法：阻断大鼠双侧颈总动脉１５ｍｉｎ和再灌注２４ｈ建立脑缺血－再灌注损伤模型，用ＮＡＤＰＨ－ｄ组化技术检测脑ＮＯＳ阻性细胞数目的变化和Ｒｈｙ的影响。结果：脑缺血－再灌注２４ｈ后，皮层和海马ＮＯＳ阳性细胞数目显著增多，Ｒｈｙ（１２．５和２５ｍｇ／ｋｇ）缺血前３０ｍｉｎ腹腔注射，可显著抑制此增多，与ＮＯＳ拮抗剂Ｌ

Effect of rhynchophylline on the change of NOS in cerebral ischemia-reperfusion rats

OBJECTIVE:To study the effect of rhynchophylline(Rhy) on the change of NOS activity in cortex and hippocampus in a rat ischemia-reperfusion model.METHOD:Cerebral ischemia-reperfusion of rats was induced by bilaterally occlusion of common cartid arteries for 15min and reperfusion for 24h.NADPH-diaphorase histochemical staining was used to detecte the NOS-positive cells.RESULTS:The numbers of NOS-positive cells in cortex and hippocampus increased significantly in ischemia-reperfusion rats,which was inhibited by Rhy(12.5 and 25mg/kg ip,30min befor ischemia).The effect of Rhy was similar to that of L-NAME,a inhibitor of NOS.CONCLUSION:Rhy may protect brain from ischemic injury by inhibition of NOS druing cerebral ischemia-reperfusion.