| 色氨酸酶基因工程菌酶法合成L-色氨酸 |
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| 引用本文： | 韦平和,吴梧桐,王晣. 色氨酸酶基因工程菌酶法合成L-色氨酸[J]. 中国现代应用药学, 1999, 16(6): 37-39 |
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| 作者姓名： | 韦平和 吴梧桐 王晣 |
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| 作者单位： | 南京,210009,中国药科大学生物制药学院 |
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| 摘 要： | 目的：以Ｌ－丝氨酸和吲哚为底物，用色氨酸酶基因工程菌酶法合成Ｌ－色氨酸。方法：以ＩＰＴＧ诱导工程菌色氨酸酶表达，将酶活最高时的工程菌游离细胞作为转化反应的酶源，通过纸层析分析并测定转化液中Ｌ－色氨酸的含量。结果：１００ｍｌ反应液（Ｌ－丝氨酸３ｇ吲哚３ｇ）３７℃反应４８ｈ可积累Ｌ－色氨酸４．９ｇ，Ｌ－丝氨酸转化率为８４．２％，吲哚转化率为９３．６％。经分离经所得的Ｌ－色酸晶体，在熔点、旋光性和红外吸
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| 关 键 词： | 色氨酸酶 基因工程菌 L－色氨酸 合成 |
Enzymatica synthesis of L-Tryptophan by tryptophanase expressed in genetic genetic engineering strain |
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| Wei Pinghe(Wei PH,Wu Wutong(Wu WT,Wang Min(Wang M. Enzymatica synthesis of L-Tryptophan by tryptophanase expressed in genetic genetic engineering strain[J]. The Chinese Journal of Modern Applied Pharmacy, 1999, 16(6): 37-39 |
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| Authors: | Wei Pinghe(Wei PH Wu Wutong(Wu WT Wang Min(Wang M |
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| Affiliation: | Wei Pinghe(Wei PH),Wu Wutong(WuWT),Wang Min(Wang M);(School of BiologicalPharmacetics,China Pharmaceutical University,Nanjing 210009) |
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| Abstract: | OBJECTIVE:L-Tryptophan was enzymatically synthesized by tryptophanase with the substrates of L-serine and indole in genetic engineering strain.METHOD:Tryptophanase was expressed in engineering strain which was induced by IPTG.The engineering strain free c |
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| Keywords: | tryptophanase genetic engineering strain L tryptophan L serine conversion |
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