一氧化氮对急性胆道感染大鼠肾功能影响作用研究

目的　探讨一氧化氮（nitricoxide,NO）对实验性急性胆道感染大鼠肾功能的影响作用。方法　Wistar大鼠35只，随机分为急性胆道感染组（AC组）、急性胆道感染加左旋精氨酸组（L组）、左旋硝基精氨酸甲基酯组（N组）、单纯胆道梗阻对照组（O组）和假手术组（SO组）5组，分别测定血浆NO、BUN、Cr值和肾组织一氧化氮合成酶（NOS）活性，并行肝、肾组织病理变化观察。结果　L组NO、NOS高于其余各组（P＜0.05），BUN、Cr低于AC组和N组（P＜0.05），而与O组比较差异无显著性意义（P＞0.05），肾组织病理变化较AC组轻；N组NO、NOS低于其余各组，而BUN、Cr则高于其余各组（P＜0.05）。结论　NO对急性胆道感染72小时大鼠的肾功能有保护作用，其作用机理可能与其舒张肾血管，增加血液灌流量等作用有关。

THE ROLE OF NITRIC OXIDEIN THE PATHOGENESIS OF RENAL DYSFUNCTION DURING ACUTE BILIARY INFECTION IN RATS

Objective To investigate the effect of nitric oxide (NO) on therenal function in acute biliary infection. Methods 35 Wistar rats were divided randomly into acute biliary infection group (AC), group of AC with L-arginine(L), group of L-NAME(N), group of simple biliary obstruction(O) and a sham-operated group(SO), and serum NO、 BUN、 Cr and NOS were determined and pathologic changes of liver and kidney were observed. Results NO and NOS were significantly higher in L group than in any other groups (P＜0.05), BUN and Cr were significantly lower than in AC and N groups (P＜0.05), but showed no significance compared with O group (P＞0.05). The pathology of kidney in L group showed a less severe change than that in AC group; NO and NOS in N group were lower than those in other groups. BUN and Cr were higher than those in other groups (P＜0.05). Conclusion NO has a protection for renal function in acute biliary infected rats the mechanism being related to its dilative effect on renal vessels and increased renal blood perfusion.