p16基因对脑胶质瘤细胞顺铂化疗敏感性的影响

目的探索ｐ１６基因在脑胶质瘤发生发展过程中的作用及其与脑胶质瘤细胞顺铂化疗敏感性的关系。方法采用脂质转染的方法．将外源野生型ｐ１６基因导入胶质瘤细胞株Ｕ２５１,观察ｐ１６基因长期稳定转染对胶质瘤细胞的作用,并筛选阳性克隆。同时以空载体质粒ＰＣＤＮＡ３为对照。免疫组化、Ｎｏｒｔｈｅｒｎ杂交检测ｐ１６基因表达,对转染后细胞生长情况、细胞周期及细胞对顺铂（ｃｉｓ－ｄｉａｍｍｉｎｅｄｉｃｈｌｏｒｏｐｌａｔｉｎｕｍ,ＣＤＤＰ）敏感性的变化进行分析。结果外源ｐ１６基因的高水平表达显著掏了胶质瘤Ｕ２５１细胞的生长,克隆形成率减少,克隆形成率减少,肿瘤细胞发生了Ｇ１期阻滞,同时,细胞对顺铂的敏感性降低,化疗药物诱导的凋亡细胞数减少。结论外源野生型ｐ１６基因可抑制胶质瘤细胞恶性增殖,同时降低Ｕ２５１细胞对顺铂的化疗敏感性。ｐ１６基因转染后对顺铂诱导凋亡作用的抑制可能是其主要机制。

Effect of p16 gene on the chemosensitivity of human glioma cell line to cis-diamminedichloroplatinum

Objective To determine the effect of p16/CDK41 on the cell growth and the chemosensitivity of human glioma cells to cis-diamminedichloroplutinum (CDDP). Methods p16 gene was transfected into human glioma cell line U251 and the expression of exogenous p16 gene was examined. The effect of exogenous p16 gene on the cell growth and chemosensitivity of U251 to CDDP were eobserved. Results Expression of exogenous p16 gene inhibited the growth of U251 dramatically. GI arrest of tumor cells was observed after transfection. however, wild type p16-positive U251 cells were less sensitive to CDDP than control cells and the number of apoptotic cells following chemotherapy was reduced. Conclusion The expression of exogenous p16 gene can inhibit the growth of glioma cells, but paradoxically, the chemosensitivity of p16-positive glioma cells to CDDP is also decreased.