喉返神经切断后甲杓肌酶组织化学变化

目的:研究单侧喉返神经(RLN)切断后,两侧甲杓肌纤维所发生的酶组织化学变化。方法:建立单侧RLN麻痹动物模型,应用琥珀酸脱氢酶染色法及显微图像分析仪,动态观察双侧甲杓肌中各型肌纤维直径的变化。结果:去RLN侧4周时各型肌纤维萎缩有意义(P<0.05);10周时,已分辨不出肌型,肌纤维呈纤溶性改变。健侧甲杓肌,4周时直径已发生代偿改变,各型肌纤维均增粗(P<0.05);10周时代偿尤为显著,其中以中间肌纤维代偿最为明显,各型肌纤维构成比没有明显变化趋势。结论:当发生单侧RLN麻痹时,健侧甲杓肌各型肌纤维均增粗,以中间肌纤维最明显,对喉的功能起到一定的代偿作用。

The study of the enzyme histochemistry changes of thyroarytenoid when one side of RLN was excised

Objective: To chronologically evaluate the changes in both sides of thyroarytenoid (TA) muscles when one side of recurrent laryngeal nerve (RLN) was cut off. Method:In 7 adult mongrel dogs, 2 dogs for control and the other 5 dogs for the following experiment: a 2 cm segment of right RLN was excised under intraper-itoneal sedation. The animals were killed at 2 ,4 ,6 ,8 ,10 week respectively and the thyroarytenoid were processed for histochemical reaction and electromicroscope. The mean muscle fiber, standard deviation and muscle fiber type composition were determined. Result:For the injured sides, denervation resulted in shrinking of all the fibers at four weeks. At ten weeks , however , all the muscles were fibrosed . For the uninjured sides (left side) , TA diameter appeared compensatory changes at four weeks, this changes were obvious at ten weeks. Variability of the fiber composition was observed throughout the study; however,there was no specific trend in changes before ten weeks. Conclusion: When the RLN was excised, the fibers in the uninjured side of thyroarytenoid appeared compensatory changes in fiber diameter, which could give rise to the recovery of the laryngeal function.