Chemotactic activity in experimental gingivitis

abstract — Forty-eight dental students were screened regarding the rate of plaque formation. The four most rapid (RPF-group) and the four slowest (SPF-group) plaque formers were selected and compared as to: (1) the development of gingivitis during a 14-day period without oral hygiene, (2) the chemotactic activity elaborated by (a) gingival crevicular material (GCM) sampled 24 h after withdrawal of oral hygiene measures and (b) 4- and 8-day-old plaques, and (3) permeability-inducing factors in 4- and 8-day-old plaques. The Gingival Index, Plaque Index and flow of gingival fluid were used for assessing plaque formation and gingivitis development. The chemotactic activity was estimated with the use of Boyden's in vitro model, and subcutaneously implanted wound chambers were used for demonstrating change in vascular permeability. It was found that: (1) individuals vary considerably with regard to the rate of formation of dental plaque, (2) gingivitis developed faster in the RPF group than in the SPF group, (3) GCM sampled already 24 h after cessation of oral hygiene yielded higher chemotactic activity in the RPF group than in the SPF group, and (4) the RPF and SPF groups did not differ from one another regarding chemotactic activity or permeability-inducing activity of equal amounts (wet weight) of plaque.