子痫前期并发脏器受累的孕妇血清及胎盘氧化应激损伤水平研究

目的:探讨分析并发不同脏器受累的子痫前期孕妇血清及胎盘氧化应激损伤水平。方法:选择2012年12月—2014年12月于福建医科大学附属协和医院产科住院分娩的30例子痫前期并发脏器受累的孕妇作为研究组,30例子痫前期未并发脏器受累的孕妇作为对照1组,另选同期正常晚期妊娠孕妇30例作为对照2组。应用紫外比色法检测血清及胎盘组织中谷胱甘肽过氧化物酶(GPX)活性、过氧化氢酶(CAT)活性、脂质过氧化终产物丙二醛(MDA)水平;WST法(Total Superoxide Dismutase Assay Kit with WST-1)检测血清及胎盘组织中超氧化物歧化酶(SOD)的活性水平。结果:1研究组及对照1组孕妇血清及胎盘组织中GPX、CAT、SOD活性水平均低于对照2组,且研究组均低于对照1组,差异均有统计学意义(P0.05)。2研究组及对照1组孕妇血清及胎盘组织中MDA水平均高于对照2组,且研究组高于对照1组,差异均有统计学意义(P0.05)。3研究组及对照1组孕妇血清及胎盘组织中MDA水平与GPX、CAT及SOD活性水平均呈负相关(P0.05);对照2组孕妇血清及胎盘组织中MDA水平与GPX、CAT及SOD活性水平未见明显相关性(P0.05)。结论:血清及胎盘氧化应激损伤增强可能是子痫前期发病的重要因素,且可能促使子痫前期孕妇并发脏器受累,血清及胎盘组织中抗氧化酶活性水平下降可能是血清及胎盘氧化应激损伤水平升高的原因。

Study on Oxidative Stress Injury Levels in Serum and Placenta of Pre-eclampsia in Pregnant Women Complicated with Organ Involvement

Objective:To investigate oxidative stress injury levels in serum and placenta of pre-eclampsia ( PE ) in pregnant women complicated with organ involvement. Methods:30 PE patients complicated with organ involvement who were admitted to Fujian Medical University Union Hospital for delivery from December 2012 to December 2014 were chosen as study group, 30 patients without organ involvement were chosen as control group one, another 30 normal pregnant women at the same time were chose as control group two. Ultraviolet colorimetry was used to detect glutathione peroxidase (GPX) and catalase (CAT) activity in maternal serum and placenta, as well as the malondialdehyde (MDA) level. WST was used to detect superoxide dismutase(SOD) activity in serum and placenta. Results:①Mean maternal serum and placenta GPX,CAT and SOD activity were significantly decreased in the study group and control group one as compared to control group two, study group were also lower than the control group one, both of the difference had statistical significance (P<0.05).②Mean maternal serum and placenta MDA were significantly elevated in the study group and control group one as compared to control group two, and the study group were also higher than the control group one, both of the difference had statistical significance (P<0.05).③Mean maternal serum and placenta MDA concentration were negatively correlated with the levels of GPX ,CAT and SOD in study and control group one (P<0.05). No obvious correlation was found in the control group two. Conclusions:Serum and placenta enhanced oxidative stress damage may be an important factor in the onset of PE, and can lead to PE women concurrent organ involvement. Antioxidant enzyme activity in serum and placental tissue levels drop may be the cause of the elevated levels of serum and placenta oxidative stress injury.