| 全反式维甲酸诱导人肝癌细胞HepG2凋亡的初步研究 |
| |
| 引用本文： | 谢斌,韦文萍,周青,汪渊.全反式维甲酸诱导人肝癌细胞HepG2凋亡的初步研究[J].肿瘤学杂志,2010,16(6):456-459. |
| |
| 作者姓名： | 谢斌 韦文萍 周青 汪渊 |
| |
| 作者单位： | [1]安徽医科大学分子生物学实验室,安徽合肥230032 [2]安徽省六安市人民医院,安徽六安237005 |
| |
| 基金项目： | 安徽省自然科学基金资助项目 |
| |
| 摘 要： | 目的]研究全反式维甲酸（ATRA）体外诱导人肝癌细胞株HepG2的凋亡及其作用机制。方法]ATRA处理HepG2细胞。MTT法分析细胞增殖反应;Hoechst染色法检测细胞凋亡情况;Western blot检测细胞中caspase-9、caspase-3和NF-κB蛋白表达情况。结果]A-TRA可抑制HepG2细胞增殖并诱导细胞凋亡,可上调细胞中caspase-9、caspase-3表达水平（P〈0.05）。结论]ATRA可抑制HepG2细胞增殖并诱导细胞凋亡,其作用机制可能与cas-pase-9、caspase-3表达上调有关。
|
| 关 键 词： | 全反式维甲酸 HepG2细胞 凋亡 caspase-9 caspase-3 |
All-trans-retinoic Acid Induced Apoptosis of Human Hepatoma HepG2 Cells |
| |
| Institution: | XIE Bin,WEI Wen-ping,ZHOU Qing,et al.(1.Laboratory of Molecular Biology,Anhui Medical University,Hefei 230032,China;2.Lu’an People＇s Hospital,Lu＇an 237005,China) |
| |
| Abstract: | Purpose] To investigate all-trans-retinoic acid （ATRA） induced apoptosis of human hepatoma HepG2 cells in vitro and its mechanism.Methods] HepG2 cells line pretreated with ATRA.The proliferation of HepG2 was analyzed by MTT assay.The cell apoptosis was measured by Hoechst dye.The expressions of caspase-9,caspase-3 and NF-κB protein were detected by Western blot.Results] ATRA could inhibit proliferation and induce apoptosis of HepG2 cell,Moreover,it could up-regulated caspase-9,caspase-3 expression level.Conclusion] ATRA could inhibit proliferation and induce apoptosis of HepG2 cells and this effect maybe associated with upregulation of caspase-9 and caspase-3 expression. |
| |
| Keywords: | all-trans-retinoic acid HepG2 apoptosis caspase-9 caspase-3 |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
