大鼠局灶性脑缺血再灌注时神经细胞凋亡的动态变化

目的:观察大鼠脑缺血再灌注后神经细胞凋亡的动态变化.方法:采用健康雄性大鼠大脑中动脉阻断(MCAO)模型,阻断大脑中动脉(MCA)血流2h后再灌注,在0.5h,6h,12h,24h和48h不同时间点断头取脑,连续取脑进行TuNEL染色。结果:①假手术组、缺血再灌注组非缺血侧大脑半球未见阳性的凋亡细胞。②脑缺血再灌注早期部分神经细胞发生了凋亡。随着再灌注时间的延长,凋亡细胞数目逐渐增多,12h～24h达到高峰。③凋亡细胞主要分布在梗死灶的边缘区,梗塞灶中心区和正常区无凋亡细胞。结论:神经细胞凋亡在脑缺血再灌注损伤时起着重要作用。

Neuronal apoptosis in rats after focal cerebral ischemia followed by reperfusion

Objective: To investigate neuronal apoptosis in rats after focal cerebral ischemia followed by reperfusion. Methods: The middle cerebral artery ( MCA) of rals was occluded for 2 hours by the middle cerebral artery occluded ( MCAO) model, and reperfusion was instituted for 0. 5, 6, 12, 24 and 48 hours. At each time slices of rat brains at the level of the anterior commissure were made consecutively by means of TUNEL staining. Results: Neuronal apoptosis of cerebral tissue in false operation group and not brain ischemia side by reperfusion was negative. Apoptotic cells was partly expressed at the beginning of reperfusion. The number of apoptotic cells increased with the development of reperfusion within a certain time , and reached its perk in 12 -24 hours . Apoplosis cells mostly distributed in the border infarction area, and it wasnt expressed in the central infarction area and the normal area. Conclusion: The neuronal apoptosis may play an important role in cerebral transient focal ischemia and reperfusion.