CUG结合蛋白1在非小细胞肺癌中的表达及与预后的关系

目的探讨非小细胞型肺癌组织中CUG结合蛋白1（CUGBP1）mRNA及蛋白的表达以及与预后的关系。方法选取2009年7月至2011年4月在青岛大学附属医院胸外科接受手术治疗的57例非小细胞型肺癌患者，男32例，女25例；年龄43～74（60．6±8．9）岁。通过半定量逆转录一聚合酶链反应（RT-PCR）及免疫组织化学技术检测癌组织及周围正常组织CUGBP1的基因和蛋白表达情况。所有患者术后每2个月电话随访1次，采用肿瘤进展时间（TTP）作为观察评估指标，分析CUGBP1 mRNA及临床病理各指标与预后的关系。采用χ^2检验对CUGBP1 mRNA及蛋白的表达进行统计分析，采用COX单因素及多因素分析CUGBP1 mRNA对于肺癌预后的意义。结果CUGBP1 mRNA及CUGBP1蛋白在肺癌组织中高表达，并且与TNM分期及分化密切相关。通过单因素及多因素分析，CUGBP1 mRNAP=0．0074，HR=3．701，95％CI（1．420，9．648）]、TNM分期P〈0．0001，HR=4．043，95％CI（2．098，7．794）]及年龄（P=0．0018，HR=3．207，95％CI（1．544，6．664）]可以作为非小细胞肺癌患者的独立预后因素。结论CUGBP1 mRNA及蛋白在肺癌组织中高表达。作为非小细胞肺癌患者的独立预后因素，CUGBP1 mRNA高表达患者预后较差。

Expression CUG-binding Protein 1 and Its Relationship with Prognosis of Non-small Cell Lung Cancer

Objective To detect mRNA and protein expression of CUG-binding protein 1 （CUGBP 1 ） in non-small cell lung cancer （NSCLC）, and assess the prognostic significance of CUGBP1. Methods Fifty-seven NSCLC patients who received surgical resection at the Department of Thoracic Surgery of Affiliated Hospital of Qingdao University between July 2009 and April 2011 were enrolled in this study. There were 32 male and 25 female patients with their age of 43-74 （60.6± 8.9） years. The expressions of CUGBP 1 mRNA and protein in tumor and adjacent normal tissues were detected by semi-quantitative RT-PCR and immunohistochemistry. All the patients were followed up every 2 months after discharge via the phone. Time to progression （TTP）was used to evaluate the relationship between CUGBP1 mRNA, clinicopathological variables and prognosis. The percentage of CUGBP1 mRNA and CUGBP1 expression was correlated with clinical characteristics using χ^2 test. The prognostic significance of CUGBP1 mRNA was assessed by univariate and multivariate analysis using Cox regression analysis. Results The expressions of CUGBP1 mRNA and CUGBP1 were over-expressed in cancer tissue, and were significantly correlated with TNM-stage and differentiation. Univariate and multivariate analysis showed that CUGBP1 mRNA expression （P=-0.0074, HR=3.701, 95% CI 1.420-9.648）, TNM-stage （P 〈 0.000 1, HR= 4.043, 95% CI 2.098-7.794） and age （P=0.0018, HR=3.207, 95% CI 1.544-6.664） were independent predictors ofpostsurgical survival in NSCLC patients. Conclusions CUGBPI mRNA and CUGBP1 are over-expressed in NSCLC, and over-expression of CUGBP 1 mRNA independently predicts a shorter postsurgical survival in NSCLC patients.