干细胞因子及其受体在寻常型白癜风表皮中的表达

目的 探讨SCF/c-kit信号通路在白癜风发病中的作用。方法 采用免疫组化和RT-PCR法检测17例寻常型稳定期白癜风患者和10例正常对照标本中表皮角质形成细胞的干细胞因子表达及基底层黑素细胞c-kit的表达情况。结果 白癜风非皮损区干细胞因子、c-kit蛋白表达与正常对照无明显差异(P>0.05),皮损区干细胞因子表达显著高于正常对照皮肤(P<0.05),而c-kit表达显著低于正常对照皮肤(P<0.05)。白癜风非皮损区表皮干细胞因子、c-kit mRNA表达平均水平与正常对照近似(P>0.05);皮损区干细胞因子mRNA表达水平高于非皮损区及正常对照组差异有统计学意义(P<0.05);皮损区c-kit mRNA表达水平显著低于非皮损区及正常对照组(P<0.05)。结论 SCF/c-kit的异常表达可能与白癜风的发病有关。

The expression of stem cell factor and its receptor in epidermis of vitiligo vulgaris

Objective To study the role of stem cell factor (SCF)/c-kit pathway in the pathogenesis of vitiligo,Methods The expression of SCF and c-kit was detected by immunohistochernistry and RT-PCR in 17 patients with stable vitiligo vulgaris and 10 healthy controls.Results The protein expression of SCF and c-kit was of no difference between the non-lesional epidermis of vitiligo and the control skin (P>0.05).A significantly higher protein expression of SCF and lower protein expression of c-kit were detected in lesional epidermis of vitiligo compared with the control skin (both P<0.05),The mRNA expression of SCF and c-kit was similar between non-lesional epidermis of vitiligo and control skin (P>0.05),while the level of SCF mRNA was significantly higher in lesional epidermis of vitiligo than that in non-lesional epidermis of vitiligo and control skin (P<0.05).The level of c-kit mRNA was markedly decreased in lesional epidermis than in non-lesional epidermis and control skin(P<0.05).Conclusion The abnormal expression of SCF and c-kit may be related to the pathogenesis of vitiligo.