卵巢癌药敏试验的微量法研究

目的　探讨微量检测 ATP在卵巢癌药敏试验中应用的可行性。方法　对 35例新鲜卵巢癌组织标本进行药敏试验 ,应用 Victor 2多功能检测仪微量检测 ATP值。结果　 1Victor 2多功能检测仪微量检测 ATP标准品与发光值的关系 ,在 ATP浓度为 10 - 9～ 10 - 5m ol/ml时 ,显示极好的线性关系 ,直线方程为 Y =0 .892 X +10 .2 5 7,相关系数 r=0 .998(P<0 .0 1)。 2微量检测 ATP的变异系数平均为 7.3%。 3微量检测 ATP进行药敏试验 ,敏感性 92 .0 % ,特异性 70 .0 % ,预测准确率为 87.1%。结论　应用 Victor 2多功能检测仪微量检测 ATP进行药敏试验 ,方法稳定 ,实验成本降低 ,更为简便、快速 ,误差小 ,值得推广和普及

Application of a Micromethod in Ovarian Cancer Chemosensitivity Testing in vitro

OBJECTIVE: To assess the efficacy of microdetermination in the ATP-bioluminescence assay (ATP-CVA) of ovarian cancer chemosensitivity testing in vitro. METHODS: A micromethod ATP-CVA was employed in determining the chemosensitivity of 35 specimens of fresh ovarian cancer cells using Victor 2 Multilabel Counter. RESULTS: 1. By use of Victor 2 Multilabel Counter to determine ATP, in a range of 10(-9)-10(-5) mol/ml, a linear relationship was remarkable between the log ATP and the log luminescence. The equation was Y = 0.892X + 10.257, the correlation coefficient r was 0.998, P < 0.01. 2. The coefficient of variation obtained from ATP-CVA was 7.3%. 3. The sensitivity of the test was 92.0%, the specificity 70.0%, and the total predictive accuracy of ATP-CVA 87.1%. CONCLUSION: With the use of Victor 2 Multilabel Counter, ATP-CVA is fast, cost-effective, and easy to perform with only small error.