| 聚合酶链反应检测宫颈癌组织中HPV16DNA相关序列 |
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| 引用本文： | 周亚滨,于修平,王美清,董杰德,赵蔚明,苏汀.聚合酶链反应检测宫颈癌组织中HPV16DNA相关序列[J].山东大学学报(医学版),1993(4). |
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| 作者姓名： | 周亚滨 于修平 王美清 董杰德 赵蔚明 苏汀 |
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| 作者单位： | 山东医科大学微生物学教研室，山东医科大学微生物学教研室，山东医科大学病理解剖学教研室，山东医科大学微生物学教研室，山东医科大学微生物学教研室，山东医科大学微生物学教研室 |
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| 摘 要： | 从石蜡包埋的宫颈癌组织和宫颈上皮瘤样病变(CIN)组织中提取DNA,用聚合酶链反应体外扩增人乳头瘤病毒16型(HPV16)的E6区,然后检测组织DNA中HPV16相关序列,以探讨人乳头瘤病毒16型与宫颈癌发生的关系。共检测标本28份,阳性率为42.9%(12/28)。其中宫颈癌阳性率为47.8%(11/23),高于宫颈上皮瘤样病变的阳性率。表明人乳头瘤病毒16型与人宫颈癌的发生有十分密切的关系。
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| 关 键 词： | 乳头瘤病毒 子宫颈肿瘤 脱氧核糖核酸 聚合酶链反应 |
DETECTION OF SEQUENCE RELATED TO HUMAN PAPILLOMAVIRUS TYPE 16 DNA IN CERVICAL CANCER USING POLYMERASE CHAIN REACTION |
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| Zhou Yabin,et al.DETECTION OF SEQUENCE RELATED TO HUMAN PAPILLOMAVIRUS TYPE 16 DNA IN CERVICAL CANCER USING POLYMERASE CHAIN REACTION[J].Journal of Shandong University:Health Sciences,1993(4). |
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| Authors: | Zhou Yabin |
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| Abstract: | To discuss the relation of HPV16 and cervical cancer,the DNAs
were extracted from paraffin-embedded cervical cancer and CIN tissues.Syn-
thesised oligonucleotied primer pair was site of HPV16 E6 ORF.The related
sequence of HPV16 DNA was amplified by polymerase chain reaction in vitro.
28 specimens were examinated,12 cases were positive(positive percentage
42.9%).The result was observed that closed relation in HPV16 and cervical
cancer,high positive in cervical cancer(positive percentage 47.8%,11/23)
than CIN. |
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| Keywords: | Papillomaviruses Cervix neoplasms DNA Polymerase chain reaction |
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