| HPLC-MS2鉴定大青叶水提液中的5种化学成分 |
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| 引用本文： | 刘瑞 袁波 刘志刚 李小芩 熊志立 李发美. HPLC-MS2鉴定大青叶水提液中的5种化学成分[J]. 中药材, 2005, 28(9): 772-774 |
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| 作者姓名： | 刘瑞 袁波 刘志刚 李小芩 熊志立 李发美 |
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| 作者单位： | 沈阳药科大学药学院,沈阳110016 |
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| 摘 要： | 目的:鉴定大青叶水提液中的化学成分.方法:大青叶的水提液经HPLC分离后,收集各色谱峰的馏分进行二级质谱分析,并和胞苷、尿苷、鸟苷、黄嘌呤和次黄嘌呤对照品的水溶液进行比较.结果:大青叶水提液HPLC中的5个色谱峰分别与胞苷、尿苷、鸟苷、黄嘌呤和次黄嘌呤对照品的tR和UV光谱一致,并具有相同的二级质谱碎片离子.结论:大青叶中含有胞苷、次黄嘌呤、尿苷、黄嘌呤和鸟苷等5种成分.
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| 关 键 词： | 大青叶 胞苷 尿苷 鸟苷 黄嘌呤 次黄嘌呤 |
| 收稿时间： | 2005-04-18 |
| 修稿时间： | 2005-04-18 |
Identification of 5 Constituents of the Aqueous Extract of Isatis indigotica by HPLC-MS2 |
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| Liu Rui, Yuan Bo, Liu Zhigang, Li Xiaoqing, Xiong Zhili, Li Famei. Identification of 5 Constituents of the Aqueous Extract of Isatis indigotica by HPLC-MS2[J]. Journal of Chinese medicinal materials, 2005, 28(9): 772-774 |
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| Authors: | Liu Rui Yuan Bo Liu Zhigang Li Xiaoqing Xiong Zhili Li Famei |
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| Affiliation: | School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016 |
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| Abstract: | Objective: To identify five constituents in the aqueous extract of Isatis indigotica Fort. Methods:After separation of the aqueous extract of Isatis indigotica Fort.by HPLC,the eluates of five peaks were collected separatively and analysed by MS~2. UV spec-tra and MS~2 were compared with those of reference standards of cytidine, uridine, guanosine xanthine and hypoxanthine. Results: Each HPLC elute of the aqueous extract had same retention time, UV spectra and fragment pattern in the MS~2 spectrum as the corresponding standards. Conclusion: Five constituents of the aqueous extract of Isatis indigotica Fort.are identified as cytidine, hypoxanthine, uridine, xanthine and guanosine. |
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| Keywords: | HPLC-MS2 |
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