p16和p53抑癌基因在肾母细胞瘤中的表达及与预后关系

目的 探讨p16、p53抑癌基因在肾母细胞瘤中的表达及与临床预后的关系。方法 应用免疫组织化学方法S-P和ABC法检测p16和p53基因的表达。结果 20例肾母细胞瘤标本p16蛋白染色阳性7例，阳性率为35％；其中上皮型10例，阳性4例(占40％)；间叶型5例，阳性1例(占20％)；胚胎型3例，阳性1例(占33％)；混合型2例，阳性l例(占50％)。p53蛋白阳性染色5例，阳性率为25％。其中上皮型及混合型12例中，p53阳性2例(占16．7％)；而间叶型及胚胎型8例中，阳性3例(占37．5％)。结论对肾母细胞瘤患同时进行p16和p53检测更能准确地判断其病理性质和预后。

The Relationship of Expression of Gene p16 and p53 with Biological Behavior and Prognosis of Wilms Tumor

Objective To study the tumor supressor genes p16 and p53 which express in Wilms' tumor and to explore the relation between the expression of the two genes and the pathological grade,histological type and the prognosis.Methods Twenty species from patients with nephroblastoma were analyzed by the immunohistochemical techniques of S-P and ABC.Results p16 protein were positive in 7 cases(35%),while p53 protein were positive in 5 cases(25%).The positive rate showed a significant relation with the pathological type and prognosis.Furthemore,p16 was more expressed in epithelial type and mixed type of Wilms tumor,which indicated the prognosis was better,whereas p53 protein was found more in blastemal and stromal predominant tumors with a poor prognosis.Conclusion Combination detection of p16 and p53 is helpful to investigate the biological behavior of nephroblastoma and predict the prognosis.