| UPLC-Q-Exactive Plus Orbitrap-MS鉴定桑色素在大鼠体内的代谢产物 |
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| 引用本文： | 邓志鹏,柳佳,熊山,田海涛.UPLC-Q-Exactive Plus Orbitrap-MS鉴定桑色素在大鼠体内的代谢产物[J].中国现代应用药学,2022,39(24):3262-3266. |
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| 作者姓名： | 邓志鹏 柳佳 熊山 田海涛 |
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| 作者单位： | 山东中医药大学 |
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| 基金项目： | 基于入血活性成分追踪和代谢组学研究中药桑白皮抗高血压的药效物质基础及作用机制 |
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| 摘 要： | 目的 利用UPLC-Q-Exactive PlusSOrbitrap-MS技术对大鼠灌胃给予桑色素后体内的的主要代谢产物进行研究。方法 大鼠灌胃给予桑色素20 mg/kg后,分别收集血浆、尿液和粪便样品,采用超高压液相色谱串联高分辨质谱技术测定桑色素的体内代谢物。结果 根据一级质谱分子离子信息和二级质谱碎裂离子信息,在大鼠血浆和尿液中均发现2个葡萄糖醛酸代谢产物,在粪便中发现脱氢产物。结论 桑色素在大鼠体内的主要代谢途径为葡萄糖醛酸化反应。本研究初步阐明了桑色素在大鼠体内的代谢情况,为进一步药理作用机制研究提供依据。
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| 关 键 词： | 桑色素 代谢产物 UPLC-MS技术 |
| 收稿时间： | 2022/3/24 0:00:00 |
| 修稿时间： | 2022/12/2 0:00:00 |
Metabolite Identification of Morin in Rats by UPLC-Q-Exactive Plus Orbitrap-MS |
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| DENG Zhipeng,LIU Ji,XIONG Shan,TIAN Haitao.Metabolite Identification of Morin in Rats by UPLC-Q-Exactive Plus Orbitrap-MS[J].The Chinese Journal of Modern Applied Pharmacy,2022,39(24):3262-3266. |
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| Authors: | DENG Zhipeng LIU Ji XIONG Shan TIAN Haitao |
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| Institution: | Shandong University of Traditional Chinese Medicine |
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| Abstract: | OBJECTIVE In vivo study of the metabolites of morin in rats by UPLC-Q-Exactive plus Orbitrap-MS. METHODS Rat plasma, urine and feces samples were collected after oral administration of 20 mg/kg morin, and the potential metabolites were detected by comparing the primary and secondary mass spectra (MS) of post-dose samples with blank samples. RESULTS Two glucuronidation metabolites were observed in rat plasma and urines, and one dehydrogenation metabolite was in feces according to the MS and MS/MS information. CONCLUSION The main metabolic pathway of morin is glucuronidation in rats. This preliminary study clarified the metabolism of morin in rats, and provided the basis for further study of pharmacological mechanism. |
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| Keywords: | morin metabolites UPLC-MS technique |
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