BRCA1基因miRNA结合位点多态性与肝细胞癌术后总生存期的关联研究

目的 探讨BRCA1基因microRNA(miRNA)结合位点上的单核苷酸多态性对肝细胞癌(hepatocellular carcinoma,HCC)患者术后总生存期(overall survival,OS)的影响.方法 纳入2004年6月—2013年12月于广西医科大学附属肿瘤医院接受根治术切除的363例HCC患者为...

Association between polymorphisms in miRNA binding site of BRCA1 gene and overall survival of hepatocellular carcinoma after operation

ObjectiveTo investigate the effect of single nucleotide polymorphism(SNP)in microRNA(miRNA)binding site of BRCA1 gene on the overall survival(OS)of patients with hepatocellular carcinoma(HCC)after operation.MethodsA total of 363 HCC patients with undergone radical surgery resection in the Guangxi Medical University Cancer Hospital from June 2004 to December2013 were included.The Cox proportional hazard model and the Kaplan-Meier method were used to analysis the association between polymorphisms in miRNA binding site of BRCA1 gene and OS of patients with HCC after operation.ResultsThe SNP rs8176318(C>A)in the miRNA binding site of BRCA1 gene was significantly associated with the OS of HCC patients after operation.In the recessive model,afteradjustingforhepatitisBvirus(HBV)infection,BCLCstage,tumordiameter,tumorthrombusandotherfactors,the Cox regression analysis showed that HCC patients carrying rs8176318 AA genotypes had a significantly decreased risk of death when compared with those carrying CC/CA genotype(HR=0.675,95%CI=0.494-0.922,P=0.014).The Kaplan-Meier curve showed that the median OS of HCC patients with AA genotype was significantly longer than that with CC/CA genotype(46.1 months vs 33.8 months,P=0.049).Using the GTEX database for quantitative trait locus analysis,the rs8176318 A allele was associated with lower BRCA1 mRNA expression levels in liver tissues(P<0.001).ConclusionsBRCA1 rs8176318 polymorphism is associated with a lower survival outcomes in patients with HCC after operation,and the A allele is associated with a lower risk of death after operation,but the result needs to be verified by a multi-center study with a larger sample size.