SDC2基因甲基化对结直肠癌诊断价值的Meta分析

目的 通过Meta分析方法评价多配体蛋白聚糖2(Syndecan-2,SDC2)基因甲基化作为生物标志物诊断结直肠癌(colorectal cancer,CRC)的价值.方法 计算机检索PubMed、Cochrane Library、Embase、Web of Science、CBM、万方、知网、维普数据库,查找建库至...

The diagnosis value of SDC2 methylation for colorectal cancer:a Meta-analysis

Objective To evaluate the value of SDC2 methylation as a biomarker in the diagnosis of colorectal cancer(CRC)by Meta-analysis.Methods PubMed,Cochrane Library,Embase,Web of Science,CBM,WanFang,CNKI and VIP databases were searched for the studies on SDC2 gene methylation in CRC by Sep.1st,2020.The literatures were screened and the data were extracted strictly according to included and excluded criterion.The quality of the studies was evaluated by the QUADAS-2 standard.The threshold effect and non-threshold effect were tested,and the pooled weighted sensitivity(Sen),specificity(Spe),positive likelihood ratio(PLR),negative likelihood ratio(NLR),and diagnostic odds ratio(DOR)were calculated.The summary receiver operating characteristic curve(SROC),area under curve(AUC),likelihood ratio point diagram and Fagan diagram were drawn.Subgroup analysis and sensitivity analysis were used to explore study heterogeneity,and Deek’s funnel plot was used to evaluate published bias.Results A total of 11 studies that met the standard were included,with a total of 1419 CRC patients and 1363 healthy normal controls.The pooled Sen,Spe,PLR,NLR,DOR and AUC of SDC2 methylation in the diagnosis of CRC were 0.77(95%CI:0.65-0.86),0.94(95%CI:0.92-0.96),14.17(95%CI:9.14-21.99),0.24(95%CI:0.16-0.38),58.28(95%CI:28.25-120.20)and 0.95(95%CI:0.93-0.97),respectively.Subgroup analysis showed that the stool SDC2 methylation had significantly higher diagnostic value(pooled DOR=84.23,95%CI:55.72-127.35)than that of blood group(pooled DOR=33.27,95%CI:8.05-137.56).The Asian group had significantly higher diagnostic value(pooled DOR=76.44,95%CI:56.03-104.30)than that in the non-Asian group(pooled DOR=16.50,95%CI:2.97-91.52).The result of the Deek’s funnel chart indicated that there was no published bias(P=0.840).Conclusion SDC2 gene methylation has excellent diagnostic value for CRC.