非小细胞肺癌p16基因突变与表达的研究

pl6蛋白最初是从各种肿瘤病毒转化的细胞中鉴定出的一种CDK4相关蛋白l’］。p］6基因被发现后,已有一些文献『‘－’］对其在肺癌中出现的基因缺失和突变进行了报道,但关于原发性肺癌中p16基因的突变和表达与患者的病理及临床资料相结合的研究目前还少见报道。本文采用?..

Mutation and expression of p16 gene in non-small cell lung cancer

Objective To study the expression and mutation of p16 gene and its relationship with the carcinogenesis and advancementof non-small cell lung cancer (NSCLC). Methods Immunohistochemistry (IHC) was used to detect p16 protein and the PCR andPCR-SSCP were used in the sychronous detection of the genomic status of p16 gene in 52 samples. Results 44 samples (84.6%)t"ere positive for p16 protein. Among the tumors. the expression level of p16 was lower in poorly-differentiated adenocarcinomascompared t'}ith those in well-ditltrentiated adenocarcinomas (P<0.05). 8(15.4%) samples were negatively stained, including 7patients Ivith lymphnode metastasis, the expression rate of p16 was negatively correlated with the size of tumor and the number oflymphnode metastasis. Besides these. alterations of p16 gene were detected in 5 (4 hornozygous detections and 1 point mutation) of 7patients with p16 negative staining and with lymph-nodes melastases. Conclusions The inactivation of p16 is involved in thecarcinogenesis and advancement of NSCLC and implicates in tumor differentiation and metastasis. and it is associated mainly withthe p16 gene mutations.