| K562、HL—60细胞端粒酶催化亚单位的表达 |
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| 引用本文： | 李熙鸿,王晓阳,汪凤兰,张燕,刘柏林,贾苍松,廖清奎. K562、HL—60细胞端粒酶催化亚单位的表达[J]. 实用儿科临床杂志, 2001, 16(6): 387-388 |
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| 作者姓名： | 李熙鸿 王晓阳 汪凤兰 张燕 刘柏林 贾苍松 廖清奎 |
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| 作者单位： | 1. 四川大学华西第二医院儿科
2. 四川大学华西医学中心分子生物学研究室 |
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| 基金项目： | 国家自然科学基金资助项目(项目编号:39970836) |
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| 摘 要： | 目的 探讨RT－PCR法分析肿瘤细胞人端粒酶逆转录酶（hTERT)-mRNA表达水平的临床应用价值。方法 以β-actin为内对照，采用RT－PCR法测定K562、HL－60细胞及正常人外周血的hTERT-mRNA表达水平。结果 K562、HL－60细胞hTERT表现出高的表达水平，相反，正常人外周血无或低表达。结论 RT－PCR分析hTERT-mRNA表达的方法具有特异性、敏感性高，易定量，操作简单，快速等特点，可应用于临床。
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| 关 键 词： | 端粒酶 人端粒酶逆转录酶 逆转录－聚合酶链反应 K562 HL－60 mRNA 白血病 临床应用 |
| 文章编号： | 1003-515X(2001)06-0387-02 |
| 修稿时间： | 2001-08-15 |
Expression of human telomerase catalytic subunit in K562 and HL-60 cells |
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| LI Xihong,WANG Xiaoyang,WANG Fenglan,et al.. Expression of human telomerase catalytic subunit in K562 and HL-60 cells[J]. Journal of Applied Clinical Pediatrics, 2001, 16(6): 387-388 |
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| Authors: | LI Xihong WANG Xiaoyang WANG Fenglan et al. |
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| Affiliation: | LI Xihong,WANG Xiaoyang,WANG Fenglan,et al. Pediatric Department,the Huaxi Second Hospital of Sichuan University,Chengdu 610041,P. R. China |
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| Abstract: | Objective To study the clinical application of RT PCR to analysis of the expression levels of human telomerase catalytic subunit(hTERT) mRNA.Methods We examined the expression levels of hTERT in K562, HL 60 cells and peripheral blood (PB) specimens from 11 normal children using semi quantitative RT PCR.Results The expression of hTERT was at significantly higher levels in K562, HL 60 cells compared with PB specimens of normal children (P<0.001). hTERT expression was nearly undetectable in PB specimens.Conclusion The results suggest that RT PCR method is an ideal for detecting the expression of hTERT in leukemia cells. It is specific, sensitive, quantitative, simple and quick, and can be nsed in clinical implications. |
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