| Cyclin E Expression and Chemotherapeutic Sensitivity in Breast Cancer Cells |
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| 引用本文： | 陈剑英,王国斌. Cyclin E Expression and Chemotherapeutic Sensitivity in Breast Cancer Cells[J]. 华中科技大学学报(医学英德文版), 2006, 26(5): 565-566. DOI: 10.1007/s11596-006-0521-8 |
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| 作者姓名： | 陈剑英 王国斌 |
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| 作者单位： | Department of General Surgery Union Hospital，Huazhong University of Science and Technology，Wuhan 430022，China，Department of General Surgery，Union Hospital，Huazhong University of Science and Technology，Wuhan 430022，China |
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| 基金项目： | This project was supported by a grant from National Natural Sciences Foundation of China (No. 30400432). |
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| 摘 要： | The effects of the cyclin E expression levels on chemotherapeutic sensitivity of breast cancer cell line were explored. After the cyclin E expression was knockdown in MDA-MB-435 by RNA interference, FACS analysis and SA-p-gal staining were used to evaluate the response sensitivity of breast cancer cells to DNA damage drugs (adriamycin, etc.). Adriamycin could induce G1 arrest in cyclin E knockdown MDA-MB-435 breast cell line and increase the percentage of cell senescence in cyclin E knockdown MDA-MB-435 cells. It was suggested that cyclin E knockdown could increase the chemotherapeutic sensitivity of breast cancer cells to DNA damage drugs.
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| 关 键 词： | 病理 治疗 基因表达 化学治疗 乳腺癌 |
| 收稿时间： | 2006-08-24 |
Cyclin E expression and chemotherapeutic sensitivity in breast cancer cells |
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| Jianying Chen,Guobin Wang. Cyclin E expression and chemotherapeutic sensitivity in breast cancer cells[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 2006, 26(5): 565-566. DOI: 10.1007/s11596-006-0521-8 |
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| Authors: | Jianying Chen Guobin Wang |
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| Affiliation: | Department of General Surgery, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, China |
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| Abstract: | The effects of the cyclin E expression levels on chemotherapeutic sensitivity of breast cancer cell line were explored. After the cyclin E expression was knockdown in MDA-MB-435 by RNA interference, FACS analysis and SA-p-gal staining were used to evaluate the response sensitivity of breast cancer cells to DNA damage drugs (adriamycin, etc.). Adriamycin could induce G1 arrest in cyclin E knockdown MDA-MB-435 breast cell line and increase the percentage of cell senescence in cyclin E knockdown MDA-MB-435 cells. It was suggested that cyclin E knockdown could increase the chemotherapeutic sensitivity of breast cancer cells to DNA damage drugs. |
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| Keywords: | cyclin E DNA damage RNA interference breast cancer cell |
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