| 多参数流式细胞术在成年人急性髓系白血病免疫分型中的应用 |
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| 引用本文： | 许晓东,赵声明,郑天林,常乃柏. 多参数流式细胞术在成年人急性髓系白血病免疫分型中的应用[J]. 白血病．淋巴瘤, 2007, 18(1): 270-271. DOI: 10.3760/cma.j.issn.1009-9921.2009.05.005 |
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| 作者姓名： | 许晓东 赵声明 郑天林 常乃柏 |
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| 作者单位： | 北京医院血液科,100730; |
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| 摘 要： | 目的 探讨成年人急性髓系白血病(AML)三色流式细胞术免疫分型的特点.方法 采用CD45/SSC双参数散点图设门方法 对126例成年AML患者进行三色流式细胞术免疫分型分析.结果 髓系抗原CD13、CD33和CD117在AML各亚型中均有很高的表达,阳性率分别为86.4%、70.2%和90.4%;CD34及HLA-DR表达偏低,分别为63.5%和61.7%.约34.2%AML患者伴有淋系抗原表达,AML淋系抗原CD7和CD19表达率分别为23.6%和2.3%.结论 流式细胞术免疫分型对AML的诊断及预后判断具有重要的意义.
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| 关 键 词： | 白血病,髓样 流式细胞术 免疫表型分型 |
Study on immunophenotyping with multi-color flow cytometry in adult patients with acute myeloid leukemia |
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| XU Xiao-dong,ZHAO Sheng-ming,ZHENG Tian-lin,CHANG Nai-bai. Study on immunophenotyping with multi-color flow cytometry in adult patients with acute myeloid leukemia[J]. Journal of Leukemia & Lymphoma, 2007, 18(1): 270-271. DOI: 10.3760/cma.j.issn.1009-9921.2009.05.005 |
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| Authors: | XU Xiao-dong ZHAO Sheng-ming ZHENG Tian-lin CHANG Nai-bai |
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| Abstract: | Objective To evaluate the characteristics of immunophenotyping in adults with acute myeloid leukemia (AML) using muhi-eolor flow cytometry. Methods Immunophenotyping was performed by three color flow cytometry using CD45/SSC gating. Results In 126 patients with AML, the myeloid antigen of CD13, CD33 and CD117 was highly expressed. The positive rate was 86.4 %, 70.2 % and 90.4 %, respectively. The CD34 and HLA-DR were lowerly expressed as 63.5 % and 61.7 %, respectively. About 34.2 % of lymphoid-assoeiated antigen expression of all the AML patients. The lymphoid-associated antigens of CD7 and CD19 expression in patients with AML was 23.6 % and 2.3 %, respectively. Conclusion Multi-color flow cytometry is an important method for diagnosis and prognosis for AML. |
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| Keywords: | Leukemia myeloidFlow cytometryImmunophenotyping |
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