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多参数流式细胞术在成年人急性髓系白血病免疫分型中的应用
引用本文:许晓东,赵声明,郑天林,常乃柏. 多参数流式细胞术在成年人急性髓系白血病免疫分型中的应用[J]. 白血病.淋巴瘤, 2007, 18(1): 270-271. DOI: 10.3760/cma.j.issn.1009-9921.2009.05.005
作者姓名:许晓东  赵声明  郑天林  常乃柏
作者单位:北京医院血液科,100730;
摘    要:目的 探讨成年人急性髓系白血病(AML)三色流式细胞术免疫分型的特点.方法 采用CD45/SSC双参数散点图设门方法 对126例成年AML患者进行三色流式细胞术免疫分型分析.结果 髓系抗原CD13、CD33和CD117在AML各亚型中均有很高的表达,阳性率分别为86.4%、70.2%和90.4%;CD34及HLA-DR表达偏低,分别为63.5%和61.7%.约34.2%AML患者伴有淋系抗原表达,AML淋系抗原CD7和CD19表达率分别为23.6%和2.3%.结论 流式细胞术免疫分型对AML的诊断及预后判断具有重要的意义.

关 键 词:白血病,髓样   流式细胞术   免疫表型分型   

Study on immunophenotyping with multi-color flow cytometry in adult patients with acute myeloid leukemia
XU Xiao-dong,ZHAO Sheng-ming,ZHENG Tian-lin,CHANG Nai-bai. Study on immunophenotyping with multi-color flow cytometry in adult patients with acute myeloid leukemia[J]. Journal of Leukemia & Lymphoma, 2007, 18(1): 270-271. DOI: 10.3760/cma.j.issn.1009-9921.2009.05.005
Authors:XU Xiao-dong  ZHAO Sheng-ming  ZHENG Tian-lin  CHANG Nai-bai
Abstract:Objective To evaluate the characteristics of immunophenotyping in adults with acute myeloid leukemia (AML) using muhi-eolor flow cytometry. Methods Immunophenotyping was performed by three color flow cytometry using CD45/SSC gating. Results In 126 patients with AML, the myeloid antigen of CD13, CD33 and CD117 was highly expressed. The positive rate was 86.4 %, 70.2 % and 90.4 %, respectively. The CD34 and HLA-DR were lowerly expressed as 63.5 % and 61.7 %, respectively. About 34.2 % of lymphoid-assoeiated antigen expression of all the AML patients. The lymphoid-associated antigens of CD7 and CD19 expression in patients with AML was 23.6 % and 2.3 %, respectively. Conclusion Multi-color flow cytometry is an important method for diagnosis and prognosis for AML.
Keywords:Leukemia  myeloidFlow cytometryImmunophenotyping
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