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Molecular cloning of the brushtail possum (Trichosurus vulpecula) immunglobulin E heavy chain constant region |
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| Authors: | Belov K Harrison G A Miller R D Cooper D W |
| Institution: | a CRC for Conservation and Management of Marsupials, School of Biological Sciences, Macquarie University, North Ryde, NSW, 2109, Australia b School of Science, University of Western Sydney Nepean, PO Box 10, Kingswood, NSW, 2747, Australia c Department of Biology, University of New Mexico, Albuquerque, NM 87131-001, USA |
| Abstract: | The immunobiology of marsupial IgE is poorly understood. As a first step towards the development of immunological reagents for marsupials and to obtain a further understanding of immunoglobulin evolution, a brushtail possum (Trichosurus vulpecula) mesenteric lymph node cDNA library was screened for the heavy chain constant region of IgE (Cε), using a partial Cε probe from the American marsupial, Monodelphis domestica. The cDNA sequence for T. vulpecula Cε was determined and found to be most similar to the M. domestica Cε sequence (76%) at the amino acid level]. T. vulpecula Cε has amino acid sequence similarities ranging from 43–52% with various eutherian Cε sequences. The secondary structure of T. vulpecula Cε, based on loops formed by internal disulfide bonds, more closely resembles rodent Cε than the American marsupial sequence. |
| Keywords: | IgE Marsupial Brushtail possum Heavy chain Constant region Trichosurus vulpecula |
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